LOCUS       sequence-277285-        1322 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0814
FEATURES             Location/Qualifiers
     source          1..1322
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggttcactg tgccggctgc aaaaggccca tcctggaccg ctttctcttg
       61 aacgtgctgg acagggcctg gcacgtcaag tgcgtccagt gctgtgaatg taaatgcaac
      121 ctgaccgaga agtgcttctc cagggaaggc aaactctact gcaagaacga cttcttccgg
      181 tgtttcggta ccaaatgcgc aggctgcgct cagggcatct cccctagcga cctggtgcgg
      241 agagcgcgga gcaaagtgtt tcacctgaac tgcttcacct gcatgatgtg taacaagcag
      301 ctctccactg gcgaggaact ctacatcatc gacgagaata agttcgtctg caaagaggat
      361 tacctaagta acagcagtgt tgccaaagag aacagccttc actcggccac cacgggcagt
      421 gaccccagtt tgtctccgga ttcccaagac ccgtcgcagg acgacgccaa ggactcggag
      481 agcgccaacg tgtcggacaa ggaagcgggt agcaacgaga atgacgacca gaacctgggc
      541 gccaagcggc ggggaccgcg caccaccatc aaagccaagc agctggagac gctgaaggcc
      601 gccttcgctg ctacacccaa gcccacccgc cacatccgcg agcagctggc gcaggagacc
      661 ggcctcaaca tgcgcgtcat tcaggtctgg ttccagaacc ggcgctccaa ggagcggagg
      721 atgaagcagc tgagcgccct gggcgcccgg cgccacgcct tcttccgcag tccgcgccgg
      781 atgcggccgc tggtggaccg cctggagccg ggcgagctca tccccaatgg tcccttctcc
      841 ttctacggag attaccagag cgagtactac gggcccgggg gcaactacga cttcttcccg
      901 caaggccccc cgtcctcgca ggcccagaca ccagtggacc tacccttcgt gccgtcatct
      961 gggccgtccg ggacgcccct gggtggcctg gagcacccgc tgccgggcca ccacccgtcg
     1021 agcgaggcgc agcggtttac cgacatcctg gcgcacccac ccggggactc gcccagcccc
     1081 gagcccagcc tgcccgggcc tctgcactcc atgtcggccg aggtcttcgg acccagcccg
     1141 cccttctcgt cgctgtcggt caacggtggg gcgagctacg gaaaccacct gtcccacccc
     1201 cccgaaatga acgaggcggc cgtgtggtag taatgaacta gtccgtaact tgaaagtatt
     1261 tcgatttctt ggctttatat atcttgtgga aaggacgagt ctctgcgaag actggagtag
     1321 ct
//
