LOCUS       sequence-277291-        1310 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0816
FEATURES             Location/Qualifiers
     source          1..1310
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggcgcg cggggagctg ggcccggccc gggagtcggc gggaggcgac
       61 ctgctgctag cactgctggc gcggagggcg gacctgcgcc gagagatccc gctgtgcgct
      121 ggctgtgacc agcacatcct ggaccgcttc atcctcaagg ctctggaccg ccactggcac
      181 agcaagtgtc tcaagtgcag cgactgccac acgccactgg ccgagcgctg cttcagccga
      241 ggggagagcg tttactgcaa ggacgacttt ttcaagcgct tcgggaccaa gtgcgccgcg
      301 tgccagctgg gcatcccgcc cacgcaggtg gtgcgccgcg cccaggactt cgtgtaccac
      361 ctgcactgct ttgcctgcgt cgtgtgcaag cggcagctgg ccacgggcga cgagttctac
      421 ctcatggagg acagccggct cgtgtgcaag gcggactacg aaaccgccaa gcagcgagag
      481 gccgaggcca cggccaagcg gccgcgcacg accatcaccg ccaagcagct ggagacgctg
      541 aagagcgctt acaacacctc gcccaagccg gcgcgccacg tgcgcgagca gctctcgtcc
      601 gagacgggcc tggacatgcg cgtggtgcag gtttggttcc agaaccgccg ggccaaggag
      661 aagaggctga agaaggacgc cggccggcag cgctgggggc agtatttccg caacatgaag
      721 cgctcccgcg gcggctccaa gtcggacaag gacagcgttc aggaggggca ggacagcgac
      781 gctgaggtct ccttccccga tgagccttcc ttggcggaaa tgggcccggc caatggcctc
      841 tacgggagct tgggggaacc cacccaggcc ttgggccggc cctcgggagc cctgggcaac
      901 ttctccctgg agcatggagg cctggcaggc ccagagcagt accgagagct gcgtcccggc
      961 agcccctacg gtgtcccccc atcccccgcc gccccgcaga gcctccctgg cccccagccc
     1021 ctcctctcca gcctggtgta cccagacacc agcttgggcc ttgtgccctc gggagccccc
     1081 ggcgggcccc cacccatgag ggtgctggca gggaacggac ccagttctga cctatccacg
     1141 gggagcagcg ggggttaccc cgacttccct gccagccccg cctcctggct ggatgaggta
     1201 gaccacgctc agttctagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1261 ctttatatat cttgtggaaa ggacgattca gttcgacacg cacttcactc
//
