LOCUS       sequence-27730-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Valdembri D, Caswell PT, Anderson KI, Schwarz JP, Koenig I, Astanina
            E, Caccavari F, Norman JC, Humphries MJ, Bussolino F, Serini G
  TITLE     Neuropilin-1/GIPC1 signaling regulates alpha5beta1 integrin traffic 
            and function in endothelial cells.
  JOURNAL   PLoS Biol. 2009 Jan 27. 7(1):e25.
  PUBMED    19175293
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Biol. 
            2009 Jan 27. 7(1):e25."
COMMENT     Sequence Label: Pinco-mNrp1dSEA
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..78
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        59..77
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             314..331
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             344..370
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gtnntagcng agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta
       61 atacgactca ctatagggag acccaagctt agtgaaccgt cagatccgct agtggatcca
      121 aagaattcct aaatcaccgg ttacctccag tttccagatt gtttgaactt ctctggccgc
      181 acaatacagg aaggaaggct gccgcagctc agggacctcc agcgtctgca gcatgggctg
      241 gttcactggg attgcctgtc ttttctgggg tgtattactt acagccagag caaactatgc
      301 aaacggagga tcccatcacc atcaccatca cagatctgac ggatacccat acgacgtccc
      361 agactacgct aaatgtggcg ggaccataaa aatcgaaaac ccagggtacc tcacatctcc
      421 cggttaccct cattcttacc atccaagtga gaagtgtgaa tggctaatcc aagctccgga
      481 accctaccag agaatcatga tcaacttcaa cccacatttc gatttggagg acagagactg
      541 caagtatgac tacgtggaag taattgatgg ggagaatgaa ggcggccgcc tgtgggggaa
      601 gttctgtggg aagattgcac cttctcctgt ggtgtcttca gggccctttc tcttcatcaa
      661 atttgtctct gactatgaga cacatggggc agggttttcc atccgctatg aaatctnnan
      721 agagaggccc gaatgntnnt cnaanctata cagcacctac tgggaggtga taaaagttcc
      781 cctgggtttc ctggaa
//
