LOCUS       sequence-277309-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0830
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggtagcaa cagtgggcag gctggccgcc acatctataa atccctagct
       61 gatgatggcc cctttgactc tgtggagccg cctaaaagac ccaccagcag actcatcatg
      121 cacagcatgg ccatgttcgg aagagagttc tgctacgcgg tggaggcagc gtatgtgacc
      181 ccagtcctgc tcagcgtagg tctgcccagc agcctgtaca gcattgtgtg gttcctcagc
      241 cccatcctgg gattcctgct gcagcccgtg gtcggatcgg ccagcgacca ctgccggtcc
      301 aggtggggcc gccggagacc ctacatcctc accctgggag tcatgatgct cgtgggcatg
      361 gctctgtacc tcaatggggc tactgttgta gcagctttga ttgctaaccc aaggaggaag
      421 ctggtttggg ccataagtgt caccatgata ggtgtcgttc tctttgattt tgctgccgac
      481 ttcattgatg ggcccatcaa agcctactta tttgatgtct gctcccatca ggacaaggag
      541 aagggcctcc actaccatgc cctcttcaca gactcgcagg gcaatgacat taaagtcact
      601 gctgagagca ctggtgaaca tgcctcctca ctaccgctac ctttgcatca gccacctcat
      661 tggatggaca gccttcctgt ccaacatgct gttcttcaca gatttcatgg gccagattgt
      721 gtaccgcggg gatccctata gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
      781 tggctttata tatcttgtgg aaaggacgaa cgaggcgctg cgatgttgat cat
//
