LOCUS       sequence-277312-        1655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0832
FEATURES             Location/Qualifiers
     source          1..1655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggccct aggccctagc agccagaatg tcactgaata cgtcgttcga
       61 gttcctaaga atacaaccaa aaaatataac atcatggctt ttaatgcagc cgacaaagtc
      121 aactttgcta cgtggaatca ggctcggctg gagcgggact tgagcaacaa gaaaatctac
      181 caagaggagg agatgcccga atcgggcgcg ggcagtgagt tcaaccgcaa gcttcgggag
      241 gaggctcgga ggaagaagta cggcatcgtc ctcaaggagt tccggcccga ggaccagccc
      301 tggctgctcc gggtcaacgg caaatcaggc aggaagttca agggcatcaa gaagggaggc
      361 gtaacagaga acacgtccta ctacatcttc acccagtgcc ccgacggggc cttcgaggcc
      421 ttccccgtgc acaactggta caatttcaca ccgctggccc ggcatcgcac gctcactgcc
      481 gaggaggccg aggaggagtg ggagaggagg aacaaggtgc tgaaccactt cagcatcatg
      541 cagcagcggc ggctcaagga tcaggaccag gacgaggatg aggaggagaa ggagaaacgt
      601 ggccgcagga aggcgagcga gctgcgcatc cacgacctgg aggacgacct ggagatgtcg
      661 tccgatgcca gtgatgccag tggtgaggag gggggcagag tccccaaggc caagaagaag
      721 gcgccgctgg ccaagggcgg caggaaaaag aagaagaaga agggttcaga cgacgaggcc
      781 ttcgaggaca gcgatgatgg ggacttcgag ggccaagagg tggactacat gtcagacggc
      841 tccagtagct cccaagaaga gcctgagagc aaggccaagg cgccgcagca ggaggagggg
      901 cccaagggtg tcgatgagca gagcgacagt agtgaggaga gtgaggagga gaagccgcct
      961 gaggaggaca aggaggagga ggaggagaag aaggcaccca ccccgcagga gaagaagcgc
     1021 aggaaagaca gcagcgagga gtcggacagc tcagaggaga gcgacattga cagcgaggcc
     1081 tcctcagccc tcttcatggc gaagaagaag acgccaccca agagagagcg gaagccgtcg
     1141 ggagggagct caaggggcaa cagccgccca ggcacgccca gcgcagaggg tggcagcacc
     1201 tcctccaccc tgcgggcggc tgccagcaaa ctcgagcaag ggaagcgggt gagcgagatg
     1261 cctgcagcca agcggttgcg gctggacacg ggaccccaga gcctgtctgg gaagtcgaca
     1321 ccccagccac catcaggcaa gacaacaccc aacagcggcg acgtgcaggt gactgaggat
     1381 gccgtgcgcc gctacctgac acggaagccc atgaccacta aggacctgct gaaaaagttc
     1441 cagaccaaga agacagggct gagcagcgag cagacagtga acgtgttggc ccagatcctc
     1501 aagcgactca accccgagcg caagatgatc aacgacaaaa tgcacttctc cctcaaggag
     1561 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1621 ggaaaggacg atcactgtct gggcccgcta ggctg
//
