LOCUS       sequence-277315-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0833
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgagcg cggggaactc gacttgaccg gcgccaaaca gaacacagga
       61 gtgtggctag tcaaggttcc taaatatttg tcacagcaat gggctaaagc ctctggaaga
      121 ggtgaagttg ggaaactgcg gattgccaag actcaaggaa ggactgaggt gtcatttact
      181 ttgaatgagg atcttgcaaa tattcatgat attggtggaa aaccagcttc agtcagtgct
      241 cctagagaac atccatttgt cttgcaaagt gttggaggac agacattaac agtatttact
      301 gagagctcat cagataagct gtcattggaa ggaatagtgg tacaaagagc tgaatgccga
      361 ccagctgcca gtgaaaacta catgcgatta aaaagattgc aaatagaaga gtcttccaaa
      421 ccagtgaggc tatcacaaca gctggacaaa gttgtaacaa ccaattacaa acctgttgct
      481 aatcatcaat acaatatcga atatgaaagg aaaaagaaag aagacggaaa gcgagctcga
      541 gctgataaac aacatgtttt agacatgcta ttttcagcct ttgagaaaca tcaatactat
      601 aatcttaagg acttggtgga catcacaaag caacctgtgg tgtacctgaa ggaaatctta
      661 aaagaaattg gtgttcagaa tgtaaaaggg atccacaaaa acacatggga gctgaagcca
      721 gagtacagac actatcaagg agaagaaaag agtgactagt aatgaactag tccgtaactt
      781 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaatc atacggagat
      841 aaattattgc a
//
