LOCUS       sequence-277321-         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0839
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcacatgt ttcttcagaa actcaagatg tttcccccaa agatgaatta
       61 actgcttcag aagcctccac taggtctcca ttgtgtgaac acaccttccc tggggactca
      121 gacttacggt caatgattga agaacatgct tttcaggttt tgtcacaagg atccttgtta
      181 gaaagtccaa gttacacagt ttgtgtctct gagccagata aagatgatga ttttctttct
      241 ctgaactttc ccaggaaact ttggaaaata gtggaaagtg accaattcaa gtctatttca
      301 tgggatgaga atggaacttg catagtgatt aatgaagaac tcttcaagaa agaaattttg
      361 gaaacaaagg ctccttacag aatatttcaa actgatgcta tcaaaagttt tgttcgacag
      421 ctcaaccttt atggatttag taaaattcaa cagaattttc aaagatctgc ctttctagcc
      481 acctttctgt cagaagagaa agaatcgtct gtcttaagca agatacgctt caccaaaatg
      541 aaactttcca gatcttcaac ttatgaaaac aggtatttat gttgcaactt acatttaaaa
      601 gatgagtcga attactcata gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
      661 tggctttata tatcttgtgg aaaggacgat taaagggcct aaatagacca ttt
//
