LOCUS       sequence-277324-        1307 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0840
FEATURES             Location/Qualifiers
     source          1..1307
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcacatgt ttcttcagaa actcaagatg tttcccccaa agatgaatta
       61 actgcttcag aagcctccac taggtctcca ttgtgtgaac acaccttccc tggggactca
      121 gacttacggt caatgattga agaacatgct tttcaggttt tgtcacaagg atccttgtta
      181 gaaagtccaa gttacacagt ttgtgtctct gagccagata aagatgatga ttttctttct
      241 ctgaactttc ccaggaaact ttggaaaata gtggaaagtg accaattcaa gtctatttca
      301 tgggatgaga atggaacttg catagtgatt aatgaagaac tcttcaagaa agaaattttg
      361 gaaacaaagg ctccttacag aatatttcaa actgatgcta tcaaaagttt tgttcgacag
      421 ctcaaccttt atggatttag taaaattcaa cagaattttc aaagatctgc ctttctagcc
      481 acctttctgt cagaagagaa agaatcgtct gtcttaagca agttaaagtt ctattataat
      541 ccaaatttca agcgtggcta tccccaactt ttagtaagag tgaagagaag aattggtgtt
      601 aaaaatgctt cacctatatc tactttattc aacgaagatt tcaacaagaa gcattttaga
      661 gcaggggcta acatggagaa tcataattct gccttagctg ctgaagctag tgaagaaagt
      721 ttattttcag cctctaaaaa tttaaatatg cctctaacaa gggaatcttc tgtcagacag
      781 ataattgcaa attcatctgt ccccattaga agtggtttcc ctcctccttc accttcaacc
      841 tcagttggac catcagaaca aattgcaaca gatcaacatg ctattttaaa tcagttgacc
      901 actattcata tgcactctca tagtacctac atgcaagcaa ggggccacat tgtgaatttt
      961 attacaacca caacttctca ataccacatc atatctccct tacaaaatgg ttattttggg
     1021 ctgacagtgg aaccatctgc tgttcccaca cgatatcctc tggtatcagt caatgaggct
     1081 ccatatcgta acatgctacc agcaggcaac ccgtggttgc aaatgcctac gatcgctgat
     1141 agatcagctg cccctcattc caggctagct cttcaaccat caccactgga caaatatcac
     1201 cctaattaca actagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1261 tatatatctt gtggaaagga cgaattcgag aatcctcgta actaatg
//
