LOCUS       sequence-277327-        1937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0844
FEATURES             Location/Qualifiers
     source          1..1937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcatctac ttgggctatc caggcccaca tggaccagga tgaacctttg
       61 gaagtaaaga tagaggaaga gaaatatacc accagacagg attgggacct gcgtaaaaac
      121 aacacccata gcagagaggt cttccgtcag tacttcagac agttctgcta ccaggagaca
      181 tctggtcccc gtgaggcttt gagccgactc cgagaacttt gccatcagtg gctgaggcca
      241 gagacccaca ccaaagaaca gattctggag ctgctggtgc tggagcagtt cctgaccatc
      301 ctacctgagg agctccaggc ctgggtgcag gagcagcatc cagagagtgg ggaggaggtg
      361 gtgactgtgc tggaggattt agagagagaa ctggatgaac caggagagca ggtctcagtc
      421 cacactgggg aacaggaaat gttcttgcag gagacggtac gtctacgaaa agaaggagaa
      481 cccagtatgt ccctccagtc catgaaagcc cagccaaagt atgaatctcc agaacttgaa
      541 tcccaacagg agcaagtttt agatgttgag actggaaatg agtatgggaa tttaaagcaa
      601 gaagtttctg aagaaatgga accacatggg aagacatcca gtaaatttga aaatgatatg
      661 tccaagtctg ctaggtgtgg agaaactcgt gaacctgaag aaataacaga agagccctct
      721 gcttgctcca gagaagataa acaacctacc tgtgatgaaa atggagtaag cctgactgag
      781 aactctgacc atactgaaca tcagagaatc tgtccaggag aagaatctta cggatgtgat
      841 gactgtggaa aagcttttag tcagcactca cacctcatag aacatcagag gatccatact
      901 ggagatagac cctacaaatg cgaagaatgt ggaaaagctt tccgtgggag aactgtgctt
      961 attcgacaca aaataataca cactggagag aaaccgtata agtgtaatga atgtggcaaa
     1021 gcctttggcc ggtggtcagc tcttaaccaa catcagagac ttcacacagg agaaaaacac
     1081 tatcactgta atgactgtgg caaagccttt agtcagaaag caggcctctt tcaccatatc
     1141 aagatccaca caagagacaa accttatcag tgcactcagt gtaataaaag ttttagtcgg
     1201 cgttccatac ttactcagca tcaaggagtt cataccggcg ccaagccgta tgagtgcaac
     1261 gagtgtggaa aagcctttgt ttataactca tcccttgttt cccatcagga aatccaccac
     1321 aaagaaaaat gctatcagtg taaggaatgt gggaaatcct tcagtcagag tggccttatt
     1381 cagcatcaga gaattcacac tggggaaaaa ccctacaaat gtgacgtatg tgaaaaagcc
     1441 tttattcaaa ggacaagtct tacagaacat cagcgaattc acactggaga aagaccctat
     1501 aaatgtgata agtgtgggaa ggcgtttact caaagatcag tcctcacgga acatcagaga
     1561 atccacactg gagagaggcc ctacaagtgt gatgagtgtg ggaatgcctt ccgaggaatc
     1621 accagcctca ttcagcatca gagaatccac actggggaga agccctacca atgtgatgag
     1681 tgtggaaaag ccttcagaca gaggtcagat cttagtaaac accagagaat ccataataga
     1741 cgtggtacct atgtatgtaa agagtgtggg aaatcattca ggcagaactc agctcttact
     1801 caacatcaga ctatccacaa aggagaaaaa tctgtttctg tgtagtaatg aactagtccg
     1861 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaatcaat
     1921 cgctccacat agtggtc
//
