LOCUS       sequence-27733-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Valdembri D, Caswell PT, Anderson KI, Schwarz JP, Koenig I, Astanina
            E, Caccavari F, Norman JC, Humphries MJ, Bussolino F, Serini G
  TITLE     Neuropilin-1/GIPC1 signaling regulates alpha5beta1 integrin traffic 
            and function in endothelial cells.
  JOURNAL   PLoS Biol. 2009 Jan 27. 7(1):e25.
  PUBMED    19175293
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Biol. 
            2009 Jan 27. 7(1):e25."
COMMENT     Sequence Label: Pinco-mNrp1dCy
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             308..325
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             338..364
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agngagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttagtgaa ccgtcagatc cgctagtgga tccaaagaat
      121 tcctaaatca ccggttacct ccagtttcca gattgtttga acttctctgg ccgcacaata
      181 caggaaggaa ggctgccgca gctcagggac ctccagcgtc tgcagcatgg gctggttcac
      241 tgggattgcc tgtcttttct ggggtgtatt acttacagcc agagcaaact atgcaaacgg
      301 aggatcccat caccatcacc atcacagatc tgacggatac ccatacgacg tcccagacta
      361 cgctaaatgt ggcgggacca taaaaatcga aaacccaggg tacctcacat ctcccggtta
      421 ccctcattct taccatccaa gtgagaagtg tgaatggcta atccaagctc cggaacccta
      481 ccagagaatc atgatcaact tcaacccaca tttcgatttg gaggacagag actgcaagta
      541 tgactacgtg gaagtaattg atggggagaa tgaaggcggc cgcctgtggg ggaagttctg
      601 tgggaagatt gcaccttctc ctgtggtgtc ttcagggccc tttctcttca tcaaatttgt
      661 ctctgactat gagacacatg gggncagggt tttccatccg ctatgaaatc ttcaagagag
      721 ggcccgaatg ttctcagaac tatacagcac ctactgngtg ataaagtccc ct
//
