LOCUS       sequence-27734-e         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Valdembri D, Caswell PT, Anderson KI, Schwarz JP, Koenig I, Astanina
            E, Caccavari F, Norman JC, Humphries MJ, Bussolino F, Serini G
  TITLE     Neuropilin-1/GIPC1 signaling regulates alpha5beta1 integrin traffic 
            and function in endothelial cells.
  JOURNAL   PLoS Biol. 2009 Jan 27. 7(1):e25.
  PUBMED    19175293
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Biol. 
            2009 Jan 27. 7(1):e25."
COMMENT     Sequence Label: Pinco-mNrp1
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             304..321
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             334..360
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt agtgaaccgt cagatccgct agtggatcca aagaattcct
      121 aaatcaccgg ttacctccag tttccagatt gtttgaactt ctctggccgc acaatacagg
      181 aaggaaggct gccgcagctc agggacctcc agcgtctgca gcatgggctg gttcactggg
      241 attgcctgtc ttttctgggg tgtattactt acagccagag caaactatgc aaacggagga
      301 tcccatcacc atcaccatca cagatctgac ggatacccat acgacgtccc agactacgct
      361 aaatgtggcg ggaccataaa aatcgaaaac ccagggtacc tcacatctcc cggttaccct
      421 cattcttacc atccaagtga gaagtgtgaa tggctaatcc aagctccgga accctaccag
      481 agaatcatga tcaacttcaa cccacatttc gatttggagg acagagactg caagtatgac
      541 tacgtggaag taattgatgg ggagaatgaa ggcggccgcc tgtgggggaa gttctgtggg
      601 aagattgcac cttctcctgt ggtgtcttca gggccctttc tcttcatcan nnttgtctct
      661 gactatgaga cacatggggg caggggtttt ccatccgcta tgaaatcttc aagagagggc
      721 ccgaatgttc tcaaaaacta naaaagca
//
