LOCUS       sequence-277360-        1550 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0862
FEATURES             Location/Qualifiers
     source          1..1550
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtaatgca gtttcagggg ttagaaaatc caattcaaat tagtcctcac
       61 tgcagctgta cgccgtcagg atttttcatg gaaatgatga gtatgaagcc cgcgaaaggt
      121 gttttaacag aacaagtggc aggtcctctg ggacagaacc tggaagtgga accatactcg
      181 caatacagca atgttcagtt tccccaagtt caaccacaga tttcctcgtc atcctattat
      241 tccaacctgg gtttctaccc ccagcagcct gaagagtggt actctcctgg aatatatgaa
      301 ctcaggcgta tgccagctga gactctctac cagggagaaa ctgaggtagc agagatgcct
      361 gtaacaaaga agccccgcat gggcgcgtca gcagggagga tcaaagggga tgagctgtgt
      421 gttgtttgtg gagacagagc ctctggatac cactataatg cactgacctg tgaggggtgt
      481 aaaggtttct tcaggagaag cattaccaaa aacgctgtgt acaagtgtaa aaacgggggc
      541 aactgtgtga tggatatgta catgcgaaga aagtgtcaag agtgtcgact aaggaaatgc
      601 aaagagatgg gaatgttggc tgaatgcttg ttaactgaaa ttcagtgtaa atctaagcga
      661 ctgagaaaaa atgtgaagca gcatgcagat cagaccgtga atgaagacag tgaaggtcgt
      721 gacttgcgac aagtgacctc gacaacaaag tcatgcaggg agaaaactga actcacccca
      781 gatcaacaga ctcttctaca ttttattatg gattcatata acaaacagag gatgcctcag
      841 gaaataacaa ataaaatttt aaaagaagaa ttcagtgcag aagaaaattt tctcattttg
      901 acggaaatgg caaccaatca tgtacaggtt cttgtagaat tcacaaaaaa gctaccagga
      961 tttcagactt tggaccatga agaccagatt gctttgctga aagggtctgc ggttgaagct
     1021 atgttccttc gttcagctga gattttcaat aagaaacttc cgtctgggca ttctgaccta
     1081 ttggaagaaa gaattcgaaa tagtggtatc tctgatgaat atataacacc tatgtttagt
     1141 ttttataaaa gtattgggga actgaaaatg actcaagagg agtatgctct gcttacagca
     1201 attgttatcc tgtctccaga tagacaatac ataaaggata gagaggcagt agagaagctt
     1261 caggagccac ttcttgatgt gctacaaaag ttgtgtaaga ttcaccagcc tgaaaatcct
     1321 caacactttg cctgtctcct gggtcgcctg actgaattac ggacattcaa tcatcaccac
     1381 gctgagatgc tgatgtcatg gagagtaaac gaccacaagt ttaccccact tctctgtgaa
     1441 atctgggacg tgcagtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1501 ctttatatat cttgtggaaa ggacgaatgg cttgcagcgg agttctgagt
//
