LOCUS       sequence-277375-        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0875
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtggccac caacaccccc cacagcaccc tgagcgtcaa ctggagcctc
       61 ctgctatccc ctgagcccga tgggggcctg atggtgctcc ctaaggacag cattcagttt
      121 tcttctgccc ttgtttttac caggctgctt gagtttgaca gcaccaacgt gtccgatacg
      181 gcagcaaagc ctttgggaag accatatcct ccatactcct tggccgattt ctcttggaac
      241 aacatcactg attcattgga tcctgccacc ctgagtgcca catttcaagg ccaccccatg
      301 aacgacccta ccaggacttt tgccaatggc agcctggcct tcagggtcca ggccttttcc
      361 aggtccagcc gaccagccca accccctcgc ctcctgcaca cagcagacac ctgtcagcta
      421 gaggtggccc tgattggagc ctctccccgg ggaaaccgtt ccctgtttgg gctggaggta
      481 gccacattgg gccagggccc tgactgcccc tcaatgcagg agcagcactc catcgacgat
      541 gaatatgcac cggccgtctt ccagttggac cagctactgt ggggctccct cccatcaggc
      601 tttgcacagt ggcgaccagt ggcttactcc cagaagccgg ggggccgaga atcagccctg
      661 ccctgccaag cttcccctct tcatcctgcc ttagcatact ctcttcccca gtcacccatt
      721 gtccgagcct tctttgggtc ccagaataac ttctgtgcct tcaatctgac gttcggggct
      781 tccacaggcc ctggctattg ggaccaacac tacctcagct ggtcgatgct cctgggtgtg
      841 ggcttccctc cagtggacgg cttgtcccca ctagtcctgg gcatcatggc agtggccctg
      901 ggtgccccag ggctcatgct gctagggggc ggcttggttc tgctgctgca ccacaagaag
      961 tactcagagt accagtccat aaattagtaa tgaactagtc cgtaacttga aagtatttcg
     1021 atttcttggc tttatatatc ttgtggaaag gacgacgtgt gtcacctcgg cttcccacc
//
