LOCUS       sequence-277387-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0895
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgcggg gatgtatttg gaacattatc tggacagtat tgaaaacctt
       61 ccctttgaat tacagagaaa ctttcagctc atgagggacc tagaccaaag aacagaggac
      121 ctgaaggctg aaattgacaa gttggccact gagtatatga gtagtgcccg cagcctgagc
      181 tccgaggaaa aattggccct tctcaaacag atccaggaag cctatggcaa gtgcaaggaa
      241 tttggtgacg acaaggtgca gcttgccatg cagacctatg agatggtgga caaacacatt
      301 cggcggctgg acacagacct ggcccgtttt gaggctgatc tcaaggagaa acagattgag
      361 tcaagtgact atgacagctc ttccagcaaa gaaggccgga ctcaaaagga gaagaaagct
      421 gctcgtgctc gttccaaagg gaaaaactcg gatgaagaag cccccaagac tgcccagaag
      481 aagttaaagc tcgtgcgcac aagtcctgag tatgggatgc cctcagtgac ctttggcagt
      541 gtccacccct ctgatgtgtt ggatatgcct gtggatccca acgaacccac ctattgcctt
      601 tgtcaccagg tctcctatgg agagatgatt ggctgtgaca accctgattg ttccattgag
      661 tggttccatt ttgcctgtgt ggggctgaca accaagcctc gggggaaatg gttttgccca
      721 cgctgctccc aagaacggaa gaagaaatag taatgaacta gtccgtaact tgaaagtatt
      781 tcgatttctt ggctttatat atcttgtgga aaggacgata aaggtcgatc tttagttagt
      841 gg
//
