LOCUS       sequence-277390-        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0902
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacgcggt gttgctggag cacttccccg ggggcctaga cgcctttcct
       61 tcttcgtact tcgacgagga cgacttcttc accgaccagt cttcacggga ccccctggag
      121 gacggcgatg agctgctggc ggacgagcag gccgaggtgg agttccttag ccaccagctc
      181 cacgagtact gctaccgcga cggggcgtgc ctgctgctgc agcccgcgcc cccggccgcc
      241 ccgctagcgc tcgccccgcc gtcctcgggg ggcctcggtg agccagacga cggcggcggc
      301 ggcggctact gctgcgagac gggggcgccc ccaggcggct tcccctactc gcccggctcg
      361 ccgccctcgt gcctggccta cccgtgcgcc ggggcggcag tactgtctcc cggggcgcgg
      421 ctgcgcggcc tgagcggagc ggcggctgcg gcggcgcggc gccggcggcg ggtgcgctcc
      481 gaggcggagc tgcagcagct gcggcaggcg gccaacgtgc gcgagcggcg gcgcatgcag
      541 tccatcaacg acgccttcga ggggctgcgc tcgcacatcc ccacgctgcc ctacgagaag
      601 cgcctctcca aggtggacac gctgcgcctg gccatcggct acatcaactt cctcagcgag
      661 ctcgtgcagg ccgacctgcc cttgcgcggc ggtggcgcgg gcggctgcgg ggggccgggc
      721 ggcggcgggc gcctgggcgg ggacagcccg ggcagccagg cccagaaggt catcatctgc
      781 catcggggca cccggtcccc ctcccccagc gaccctgatt atggcctccc tcccctagca
      841 ggacactctc tctcatggac tgatgaaaaa caactcaagg aacaaaatat tatccgaaca
      901 gccaaagtct ggaccccaga ggaccccaga aaactcaaca gcaaatcttc cttcaacaac
      961 atagaaaacg aaccaccatt tgagtttgtg tcctagtaat gaactagtcc gtaacttgaa
     1021 agtatttcga tttcttggct ttatatatct tgtggaaagg acgacgtgtc cacctgtcac
     1081 tggtatct
//
