LOCUS       sequence-277408-        1346 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0924
FEATURES             Location/Qualifiers
     source          1..1346
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             887..904
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
ORIGIN
        1 accgccacca tggcaatggt agtcagcacg tggcgcgacc cccaggacga ggtgcccggc
       61 tcacagggca gccaggcctc gcaggcgccg cccgtgcccg gcccgccgcc cggcgccccg
      121 cacacgccac agacgcccgg ccaagggggc ccagccagca cgccagccca gacggcggcc
      181 ggtggccagg gcggccctgg cggcccgggt agcgacaagc agcagcagca gcaacacatc
      241 gagtgcgtgg tgtgcggaga caagtcgagc ggcaagcact acggccagtt cacgtgcgag
      301 ggctgcaaga gcttcttcaa gcgcagcgtg cggaggaacc tgagctacac gtgccgcgcc
      361 aaccggaact gtcccatcga ccagcaccat cgcaaccagt gccagtactg ccgcctcaaa
      421 aagtgcctca aagtgggcat gagacgggaa gcggtgcaga ggggcaggat gccgccgacc
      481 cagccgaccc acgggcagtt cgcgctgacc aacggggatc ccctcaactg ccactcgtac
      541 ctgtccggat atatttccct gctgttgcgc gcggagccct atcccacgtc gcgcttcggc
      601 agccaatgca tgcagcccaa caacatcatg ggtatcgaga acatttgcga actggccgcg
      661 aggatgctct tcagcgccgt cgagtgggcc cggaacatcc ccttcttccc cgacctgcag
      721 atcacggacc aggtggccct gcttcgcctc acctggagcg agctgtttgt gttgaatgcg
      781 gcgcagtgct ccatgcccct ccacgtcgcc ccgctcctgg ccgccgccgg cctgcatgct
      841 tcgcccatgt ccgccgaccg ggtggtcgcc tttatggacc acatacggat cttccaagag
      901 caagtggaga agctcaaggc gctgcacgtt gactcagccg agtacagctg cctcaaggcc
      961 atagtcctgt tcacctcaga tgcctgtggt ctctctgatg tagcccatgt ggaaagcttg
     1021 caggaaaagt ctcagtgtgc tttggaagaa tacgttagga gccagtaccc caaccagccg
     1081 acgagattcg gaaagctttt gcttcgcctc ccttccctcc gcaccgtctc ctcctcagtc
     1141 atagagcaat tgtttttcgt ccgtttggta ggtaaaaccc ccatcgaaac cctcatccgg
     1201 gatatgttac tgtccggcag cagttttaac tggccgtata tggcaattca atagtaatga
     1261 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1321 gattactgcg taaccaggat gatcaa
//
