LOCUS       sequence-277438-        1193 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0961
FEATURES             Location/Qualifiers
     source          1..1193
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtgggcag cccagacacc gttgggatga actacggcag ctacatggag
       61 gagaagcaca tgccaccccc aaacatgacc acgaacgagc gcagagttat cgtgccagca
      121 gatcctacgc tatggagtac agaccatgtg cggcagtggc tggagtgggc ggtgaaagaa
      181 tatggccttc cagacgtcaa catcttgtta ttccagaaca tcgatgggaa ggaactgtgc
      241 aagatgacca aggacgactt ccagaggctc acccccagct acaacgccga catccttctc
      301 tcacatctcc actacctcag agagactcct cttccacatt tgacttcaga tgatgttgat
      361 aaagccttac aaaactctcc acggttaatg catgctagaa acacagattt accatatgag
      421 ccccccagga gatcagcctg gaccggtcac ggccacccca cgccccagtc gaaagctgct
      481 caaccatctc cttccacagt gcccaaaact gaagaccagc gtcctcagtt agatccttat
      541 cagattcttg gaccaacaag tagccgcctt gcaaatccag gcagtggcca gatccagctt
      601 tggcagttcc tcctggagct cctgtcggac agctccaact ccagctgcat cacctgggaa
      661 ggcaccaacg gggagttcaa gatgacggat cccgacgagg tggcccggcg ctggggagag
      721 cggaagagca aacccaacat gaactacgat aagctcagcc gcgccctccg ttactactat
      781 gacaagaaca tcatgaccaa ggtccatggg aagcgctacg cctacaagtt cgacttccac
      841 gggatcgccc aggccctcca gccccacccc ccggagtcat ctctgtacaa gtacccctca
      901 gacctcccgt acatgggctc ctatcacgcc cacccacaga agatgaactt tgtggcgccc
      961 caccctccag ccctccccgt gacatcttcc agtttttttg ctgccccaaa cccatactgg
     1021 aattcaccaa ctgggggtat ataccccaac actaggctcc ccaccagcca tatgccttct
     1081 catctgggca cttactacta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1141 tggctttata tatcttgtgg aaaggacgag gcctcacctg agttgctagg gcg
//
