LOCUS       sequence-277465-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0979
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacgtgtt ggctagttat agtatattcc aggagctaca acttgtccac
       61 gacaccggct acttctcagc tttaccatcc ctggaggaga cctggcagca gacatgcctt
      121 gaattggaac gctacctaca gacggagccc cggaggatct cagagacctt tggtgaggac
      181 ttggactgtt tcctccacgc ttcccctccc ccgtgcattg aggaaagctt ccgtcgctta
      241 gaccccctgc tgctccccgt ggaagcggcc atctgtgaga agagctcggc agtggacatc
      301 ttgctctctc gggacaagtt gctatctgag acctgcctca gcctccagcc ggccagctct
      361 tctctagaca gctacacagc cgtcaaccag gcccagctca acgcagtgac ctcattaacg
      421 cccccatcgt cccctgagct cagccgccat ctggtcaaaa cctcacaaac tctctctgcc
      481 gtggatggca cggtgacgtt gaaactggtg gccaagaagg ctgctctcag ctccgtaaag
      541 gtgggagggg tcgcaacagc tgcagcagcc gtgacggctg cgggggccgt taagagtgga
      601 cagagcgaca gtgaccaagg agggctgggg gctgaagcat gtcccgaaaa caagaagagg
      661 gttcaccgct gtcagtttaa cgggtgccgg aaagtttata caaaaagctc ccacttaaag
      721 gcccaccaga ggactcacac aggtgagaag ccttataagt gctcatggga gggatgtgag
      781 tggcgttttg cacgaagcga tgagctcacg aggcactaca ggaaacacac aggtgcaaag
      841 cccttcaaat gcaaccactg cgacaggtgt ttttccaggt ctgaccatct tgccctccac
      901 atgaagagac atatctagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
      961 ctttatatat cttgtggaaa ggacgaacga atactggtga tacatcagca
//
