LOCUS       sequence-277471-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0981
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacgtgct ccccatgtgc agcatcttcc aggagctcca gatcgtgcac
       61 gagaccggct acttctcggc gctgccgtct ctggaggagt actggcaaca gacctgccta
      121 gagctggaac gttacctcca gagcgagccc tgctatgttt cagcctcaga aatcaaattt
      181 gacagccagg aagatctgtg gaccaaaatc attctggctc gggagaaaaa ggaggaatcc
      241 gaactgaaga tatcttccag tcctccagag gacactctca tcagcccgag cttttgttac
      301 aacttagaga ccaacagcct gaactcagat gtcagcagcg aatcctctga cagctccgag
      361 gaactttctc ccacggccaa gtttacctcc gaccccattg gcgaagtttt ggtcagctcg
      421 ggaaaattga gctcctctgt cacctccacg cctccatctt ctccggaact gagcagggaa
      481 ccttctcaac tgtggggttg cgtgcccggg gagctgccct cgccagggaa ggtgcgcagc
      541 gggacttcgg ggaagccagg tgacaaggga aatggcgatg cctcccccga cggcaggagg
      601 agggtgcacc ggtgccactt taacggctgc aggaaagttt acaccaaaag ctcccacttg
      661 aaagcacacc agcggacgca cacaggagaa aagccttaca gatgctcatg ggaagggtgt
      721 gagtggcgtt ttgcaagaag tgatgagtta accaggcact tccgaaagca caccggggcc
      781 aagcctttta aatgctccca ctgtgacagg tgtttttcca ggtctgacca cctggccctg
      841 cacatgaaga ggcacctcta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
      901 tggctttata tatcttgtgg aaaggacgaa ttggtgtggt cggagggctt tgc
//
