LOCUS       sequence-277510-        1421 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1086
FEATURES             Location/Qualifiers
     source          1..1421
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagaccc agatgtcctc actgaagttc cagcagcatt gaagcggtta
       61 gccaagtatg tgatccgggg attttatggc attgagcatg ccttggcctt ggacatcttg
      121 atcaggaact cctgtgtgaa agaggaggat atgctggagc tgctcaagtt tgatcggaag
      181 caacttcgat cagttttgaa taatttaaag ggagacaagt ttatcaaatg cagaatgagg
      241 gtagagactg ctgcagacgg gaaaaccact cgccataact actacttcat caattatcgt
      301 actcttgtta atgtggtaaa atataaactg gaccacatga gaagaagaat tgagaccgat
      361 gagagagatt cgaccaaccg ggcttccttc aaatgtcctg tctgtagtag tactttcaca
      421 gacttagaag ctaatcagct ctttgatcct atgacaggaa ctttccgctg tactttttgc
      481 catacagagg tagaagagga tgaatcagca atgcccaaaa aagatgcacg cacacttttg
      541 gcaaggttta atgaacaaat tgagcccatt tatgcattgc ttcgggagac agaggatgtg
      601 aacttggcct atgaaatact tgagccagaa cccacagaaa tcccagccct gaaacagagc
      661 aaggaccatg cagcaactac tgctggagct gctagcctag caggtgggca ccaccgggaa
      721 gcatgggcca ccaaaggtcc ttcctatgaa gacttataca ctcagaatgt tgtcattaac
      781 atggatgacc aagaagatct tcatcgagcc tcactggaag ggaaatctgc caaagagagg
      841 cctatttggt tgagagaaag cactgtccaa ggggcatatg gttctgaaga tatgaaagaa
      901 gggggcatag atatggacgc atttcaggag cgtgaggaag gccatgctgg gcctgatgac
      961 aacgaagagg tcatgcgagc actgctcatt cacgagaaaa agacttcctc tgccatggct
     1021 ggttcagtgg gggcagctgc tccagtgacc gctgccaatg gcagtgactc agaaagcgag
     1081 accagtgagt cagatgatga ttctccaccc cgtccggcag ctgtggctgt gcataaacga
     1141 gaagaggatg aagaggaaga tgacgagttt gaagaagtag cagatgaccc cattgtcatg
     1201 gtggctggcc gtccgttctc ctacagtgaa gtgagccaac ggccagagct agtggcccag
     1261 atgacaccag aagaaaagga agcatatata gcaatgggac aacgcatgtt tgaggacctc
     1321 tttgagtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1381 tcttgtggaa aggacgatag tagttctcga actgccggcg c
//
