LOCUS       sequence-277513-        1607 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1087
FEATURES             Location/Qualifiers
     source          1..1607
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggcttc gcgctgccgg ctcagtccca gcggcgacag tgtcttccat
       61 gaagaaatga tgaagatgcg acaggctaag ctggattatc agaggctact acttgagaag
      121 aggcaaagga aaaagcgcct tgagccattt atggtgcagc ccaatccaga agccaggcta
      181 cgtcgggcaa agccaagggc cagtgatgag cagactccct tggtgaactg tcatactccc
      241 cacagcaatg tcatcttaca tggtattgat ggtccagctg ctgtcctgaa accagacgaa
      301 gttcatgctc catcagtaag ctcctctgtt gtggaagaag atgctgaaaa caccgtggat
      361 actgcttcca agccaggact tcaggagcgt ctccaaaagc atgatatctc tgaaagtgtg
      421 aacttcgatg aggagactga tggaatatcc cagtcagcat gtttagaaag acccaattct
      481 gcatcaagcc agaattcaac cgatacaggc acttccggtt ctgctactgc cgcccaacca
      541 gctgataacc tcctgggaga catagacgac ctggaggact ttgtgtatag tcctgcccct
      601 caaggtgtca cagtaagatg tcggataatc cgggataaaa ggggaatgga tcggggtctc
      661 ttccccacct actatatgta cttggaaaaa gaagaaaatc agaagatatt tcttcttgca
      721 gctagaaagc ggaaaaagag caaaacagcc aactacctta tctccattga tccagttgat
      781 ttatctcgtg aaggagaaag ttatgtcggc aagcttagat ccaacctcat ggggaccaag
      841 tttacagttt atgaccgtgg catctgcccc atgaagggcc ggggtttggt aggagcggcc
      901 cacacccggc aggagctggc tgccatctcc tatgaaacaa acgtacttgg atttaaaggt
      961 cctaggaaaa tgtctgtgat cattcctgga atgacactga atcataagca gatcccctat
     1021 cagccacaaa acaaccatga cagtttgctc tcaaggtggc agaacagaac tatggaaaat
     1081 ctggttgagc tgcacaacaa ggcccccgtc tggaacagtg acactcagtc ctatgtcctc
     1141 aacttccgtg gccgggtcac tcaggcgtct gtgaagaact tccagatagt ccacaaaaat
     1201 gaccctgatt atatagtcat gcagtttgga cgtgtggcag atgacgtgtt cacactggat
     1261 tacaactacc cactttgtgc agtacaggcc tttggcatcg gtctttctag ctttgacaaa
     1321 tgtatccaaa ccttgagaat gcaggagctc tgtgagctcc accgtcagca ccattcagct
     1381 gcatcccttg tgcacagaac tgtttgccag cgttgggtgg gacacccgtg gcggctgctc
     1441 cctcagacct cccttctgtg gactgacctc tcacctccgc ctgttgttcc tgcaccacat
     1501 cagataagca tgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1561 tatatatctt gtggaaagga cgatatagta cgagggcgca taacgcg
//
