LOCUS       sequence-277516-        1616 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1098
FEATURES             Location/Qualifiers
     source          1..1616
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcctgggt gctcaagatg gacgaggtga tcgagtccgg gctggtgcac
       61 gacttcgacg ccagcctctc gggcatcggg caggaactgg gcgccggcgc ttacagcatg
      121 agtgatgtct tggcattgcc cattttcaag caggaagatt ccagccttcc attggatggt
      181 gaaacagagc acccaccctt tcagtatgtg atgtgtgctg caacgtcacc agcagtaaaa
      241 ctgcatgatg aaacgcttac ttatttgaac caaggtcagt catatgaaat tcggatgctg
      301 gataatcgga aaatgggtga tatgcctgag atcaatggaa aattagtaaa gagcatcata
      361 agggttgtat tccatgacag acggctacaa tacacagagc atcagcaact tgaaggatgg
      421 aagtggaatc gcccaggaga cagacttctt gatttagata ttccaatgtc tgtgggaata
      481 attgacacaa ggacgaatcc aagccagtta aatgcggttg aatttctgtg ggacccagca
      541 aaacgcacct ctgctttcat tcaggtacac tgcatcagca cagaatttac tccacggaag
      601 cacggaggtg aaaagggagt gccctttagg atccaggttg acacctttaa gcagaatgaa
      661 aatggagaat acacagatca tctacactca gctagctgcc aaatcaaagt ttttaagcct
      721 aaaggtgcag acaggaaaca aaaaactgac cgagagaaga tggagaagag aacagctcat
      781 gaaaaagaaa agtatcagcc gtcctatgat accacaatcc tcacagagtg ttctccgtgg
      841 cccgatgccc ccacagccta tgtgaataac agcccttccc cagcgcccac tttcacctcc
      901 ccacagcaga gcacttgcag tgtcccagac agcaattctt cttccccaaa tcatcaggga
      961 gatggagctt cacagacctc tggtgaacaa attcagcctt cagctacgat ccaggaaaca
     1021 cagcaatggc tgctcaaaaa cagattctct tcctacacaa gactgttctc taatttttca
     1081 ggtgccgact tattaaaact gacaaaggag gatttagttc aaatttgtgg tgcagccgat
     1141 ggaattcggc tctataattc actgaagtca aggtcggtta gaccccgttt aaccatctat
     1201 gtctgccggg agcagccaag cagcacagtg ctgcaagggc agcagcaagc tgcaagcagt
     1261 gcaagcgaga atggcagtgg ggcaccctat gtttatcatg caatctactt ggaagaaatg
     1321 attgcctcag aagttgctcg aaaacttgcg ctggtgttta atatccctct ccaccaaatt
     1381 aatcaggttt acagacaggg tcccaccggt attcacattc ttgttagtga tcagatggtt
     1441 cagaattttc aagatgagag ttgtttttta ttctccacag taaaagctga aagtagtgat
     1501 ggcatccaca taattttgaa gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1561 tcttggcttt atatatcttg tggaaaggac gactgagatt tcgcacgtta acttgg
//
