LOCUS       sequence-277534-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1145
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaagatta taccaaaata gagaaaattg gagaaggtac ctatggagtt
       61 gtgtataagg gtagacacaa aactacaggt caagtggtag ccatgaaaaa aatcagacta
      121 gaaagtgaag aggaaggggt tcctagtact gcaattcggg aaatttctct attaaaggaa
      181 cttcgtcatc caaatatagt cagtcttcag gatgtgctta tgcaggattc caggttatat
      241 ctcatctttg agtttctttc catggatctg aagaaatact tggattctat ccctcctggt
      301 cagtacatgg attcttcact tgttaaggta gtaacactct ggtacagatc tccagaagta
      361 ttgctggggt cagctcgtta ctcaactcca gttgacattt ggagtatagg caccatattt
      421 gctgaactag caactaagaa accacttttc catggggatt cagaaattga tcaactcttc
      481 aggattttca gagctttggg cactcccaat aatgaagtgt ggccagaagt ggaatcttta
      541 caggactata agaatacatt tcccaaatgg aaaccaggaa gcctagcatc ccatgtcaaa
      601 aacttggatg aaaatggctt ggatttgctc tcgaaaatgt taatctatga tccagccaaa
      661 cgaatttctg gcaaaatggc actgaatcat ccatatttta atgatttgga caatcagatt
      721 aagaagatgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
      781 atatcttgtg gaaaggacga gctagaaaca ctggtcaggc caag
//
