LOCUS       sequence-277537-         515 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1149
FEATURES             Location/Qualifiers
     source          1..515
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             202..225
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 accgccacca tggacaatgc aagaatgaac tccttcctgg aataccccat acttagcagt
       61 ggcgactcgg ggacctgctc agcccgagcc tacccctcgg accataggat tacaactttc
      121 cagtcgtgcg cggtcagcgc caacagttgc ggcggcgacg accgcttcct agtgggcagg
      181 ggggtgcaga tcggttcgcc ccaccaccac caccaccacc accatcgcca cccccagccg
      241 gctacctacc agacttccgg gaacctgggg gtgtcctact cccactcaag ttgtggtcca
      301 agctatggct cacagaactt cagtgcgcct tacagcccct acgcgttaaa tcaggaagca
      361 gacccaccaa gaagcctgtc gctcccccgc atcggagaca tcttctccag cgcagacttt
      421 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
      481 ggaaaggacg atttgactat aagccgtcat aacag
//
