LOCUS       sequence-27754-e         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  TITLE     TRONO lab plasmids
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAlbumin
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     183..200
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    317..338
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(353..358,359..376,377..383)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    391..407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     396..418
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     415..431
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     415..431
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     450..470
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        452..470
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        498..724
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 tcatgcccac acaaatctct ccctggcatt gttgtctttg cagatgtcag tgaaagagaa
       61 ccagcagctc ccatgagtcc caagctatgt tctttcctgc gttatcccct gattctgtgg
      121 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
      181 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
      241 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
      301 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
      361 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
      421 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
      481 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
      541 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
      601 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
      661 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
      721 aaacgggtc
//
