LOCUS       sequence-277543-         614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1195
FEATURES             Location/Qualifiers
     source          1..614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggaccc gaaccctgaa gagaacatga agcagcagga ttcacccaag
       61 gagagaagtc cccagagccc aggaggcaac atctgccacc tgggggcccc gaagtgcacc
      121 cgctgcctca tcaccttcgc agattccaag ttccaggagc gtcacatgaa gcgggagcac
      181 ccagcggact tcgtggccca gaagctgcag ggggtcctct tcatctgctt cacctgcgcc
      241 cgctccttcc cctcctccaa agccctaatc acccaccagc gcagccacgg tccagccgcc
      301 aagcccaccc tgccggttgc aaccactact gcccagccca ccttcccttg tcctgactgt
      361 ggcaagacct ttgggcaggc tgtttctctg aggcggcacc gccagatgca tgaggtccgt
      421 gcccctcctg gcaccttcgc ctgcacagag tgcggtcagg actttgctca ggaagcaggg
      481 ctgcatcaac actacattcg gcatgcccgg ggggagctct agtaatgaac tagtccgtaa
      541 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga gcctggcgac
      601 acctagattc cata
//
