LOCUS       sequence-277552-        1520 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1245
FEATURES             Location/Qualifiers
     source          1..1520
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1246..1255
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgatgcaaca tgcctcccca gcccccgctc tgacgatgat ggccacgcag
       61 aatgtcccgc ccccacccta ccaggacagc ccacagatga cggcaaccgc ccagccaccc
      121 tccaaggccc aggctgtcca catctctgcc ccctcagctg ctgccagcac acctgtgccc
      181 agtgccccca tcgaccccca ggcccagctg gaggctgaca agcgagctgt atacaggcac
      241 cctcttttcc cgctcctgac gctgctgttt gagaaatgtg aacaggccac ccagggctct
      301 gagtgcatca cctccgccag ctttgatgtg gacatcgaga actttgtcca ccagcaggaa
      361 caggagcaca aacccttctt cagcgatgac ccagaactgg acaatctgat ggtgaaggca
      421 atccaggtcc tgagaatcca cctgctggag ctggagaaag tcaatgaact ctgcaaggac
      481 ttttgtaacc gttacatcac ctgcctcaaa accaagatgc acagcgacaa cctgctcagg
      541 aatgatctag gggggcccta ctcccccaac cagccctcca tcaaccttca ctcacaggac
      601 ctcctgcaga attcccccaa ttccatgtcc ggagtctcca ataaccccca ggggattgtg
      661 gtcccagcct cagcgctcca gcagggcaac atcgccatga caaccgtcaa ctcacaagtt
      721 gtgtcaggtg gagccttata ccaaccggtt accatggtaa cctcccaggg tcaggtggtc
      781 acccaagcaa tcccccaggg agccatccag atccagaaca cacaggttaa ccttgacctc
      841 acctccctcc tggacaatga ggataagaag tccaagaaca aacgaggagt cttgcccaag
      901 catgccacca atataatgcg ttcttggctc ttccagcatc tcatgcaccc ctaccccacg
      961 gaggatgaga agaggcagat cgcagcccag accaacctca ccctcctgca agtaaacaac
     1021 tggttcatca atgcccggag gcgcatcctg cagcccatgc ttgatgccag caacccagat
     1081 cctgccccca aagccaagaa gatcaagtct cagcaccggc ccacccaaag attctggccc
     1141 aactccatcg ctgcgggggt gctgcagcag cagggcggtg ccccagggac aaaccccgat
     1201 ggttccatca acttggacaa cctgcagtcc ctgtcctcag acagtgccac catggccatg
     1261 cagcaggcta tgatggctgc acacgatgac tcattggatg ggacagaaga agaggatgag
     1321 gatgagatgg aagaggagga ggaggaggag ctggaggagg aggtcgacga gctgcagacg
     1381 acaaatgtca gcgacctggg cttggaacac agtgactccc tggagtagta atgaactagt
     1441 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgactac
     1501 cttctattag tgtctccagc
//
