LOCUS       sequence-277558-        1694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1289
FEATURES             Location/Qualifiers
     source          1..1694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1114..1123
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgactcc cgacgtgagt gtgcacatgg aggaggtggt ggttgtgaca
       61 actccggaca ctgcagtgga cggcagtggt gtggaggggg tgaagaccgt gttggtgacg
      121 accaacttgg cccctcacgg gggcgacctg acggaagata acatggagac agaaaatgca
      181 gcggcagcag ctgccgcggc cttcacagcc tcctcccagc tcaaggaagc cgtgttagtg
      241 aagatggctg aagaggggga gaacctggaa gctgagattg tgtaccccat cacctgtgga
      301 gacagcagag ccaacctcat ctggaggaag tttgtgtgtc ccggcatcaa tgtgaaatgt
      361 gttcagtacg acgagcatgt aatcagccca aaggaatttg tgcacctggc cgggaagtcc
      421 accctgaagg actggaagag agccatccgc atgaacggca tcatgctcag gaagatcatg
      481 gactccgggg aactggactt ctaccagcat gacaaggtct gctccaacac ctgccgcagc
      541 acaaagattg acctctcagg agcccgtgtg tccctgagca gccccacgtc ggccgagtac
      601 attcccctca cgcccgccgc agccgacgtg aatgggtctc ctgcgaccat caccatagag
      661 acctgtgaag accctggcga ctggaccgcg gccattggag atgacacatt taccttctgg
      721 cgggggctga aggacgccgg cctgctggac gaggtcatcc aggagttcca ccaggagctg
      781 gtggagacca tgagaggcct gcagcagcgg gtccaggacc ctcccctgca gcttcgagat
      841 gctgtacttc tcaacaacat cgtgcagaac ttcggcatgc tggacctggt gaagaaggtg
      901 ctggccagcc acaagtgcca gatggaccgc tcgcgggagc agtacgcccg ggacctggca
      961 gccctggagc agcagtgtga tgagcatcgt cgccgagcca aggagctgaa gcacaagtcc
     1021 cagcacctca gcaacgtgct catgacgctg acgccggtct ccctgccacc gcctgtgaag
     1081 cggccccggc ttgcacgtgc cacatcagga ccggccgcca tggcctcgca ggtgctcacc
     1141 cagtctgccc agctggcgct tggcccgggc gtgcccgtcc cccagctgac cagcgtgccc
     1201 cttggtaaag tggtgtccac cctgccgtcc accgtcctgg gcaagggttc ccttcaggcg
     1261 ccccccgcca gctccccggc ctccccgctg ctcgggggat acacagtctt ggcctcttcc
     1321 ggctccacct accccagcac agtggagatc cacccggacg cgtccagcct cacggtcctg
     1381 agcacggccg ccgtgcagga cggtagcacg gtgttcaagg tggtcagccc gctacagctg
     1441 ctcacgttgc ctggcctggg ccccaccctg cagaacgtgg cccaggcctc gcctggctcc
     1501 agcacaattg tgacagtgcc cgcaggggct gcccccgggc ctgaggagca cacggccacc
     1561 attgaggtgg ctgccatggc agaggaccac gagcggaagt agtaatgaac tagtccgtaa
     1621 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga gctatcgatt
     1681 gagttcggga ggcc
//
