LOCUS       sequence-277570-        1352 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1326
FEATURES             Location/Qualifiers
     source          1..1352
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtggacca cttacttcca gtggacgaga acttctcgtc gccaaaatgc
       61 ccagttgggt atctgggtga taggctggtt ggccggcggg catatcacat gctgccctca
      121 cccgtctctg aagatgacag cgatgcctcc agcccctgct cctgttccag tcccgactct
      181 caagccctct gctcctgcta tggtggaggc ctgggcaccg agagccagga cagcatcttg
      241 gacttcctat tgtcccaggc cacgctgggc agtggcgggg gcagcggcag tagcattggg
      301 gccagcagtg gccccgtggc ctgggggccc tggcgaaggg cagcggcccc tgtgaagggg
      361 gagcatttct gcttgcccga gtttcctttg ggtgatcctg atgacgtccc acggcccttc
      421 cagcctaccc tggaggagat tgaagagttt ctggaggaga acatggagcc tggagtcaag
      481 gaggtccctg agggcaacag caaggacttg gatgcctgca gccagctctc agctgggcca
      541 cacaagagcc acctccatcc tgggtccagc gggagagagc gctgttcccc tccaccaggt
      601 ggtgccagtg caggaggtgc ccagggccca ggtgggggcc ccacgcctga tggccccatc
      661 ccagtgttgc tgcagatcca gcccgtgcct gtgaagcagg aatcgggcac agggcctgcc
      721 tcccctgggc aagccccaga gaatgtcaag gttgcccagc tcctggtcaa catccagggg
      781 cagaccttcg cactcgtgcc ccaggtggta ccctcctcca acttgaacct gccctccaag
      841 tttgtgcgca ttgcccctgt gcccattgcc gccaagcctg ttggatcggg acccctgggg
      901 cctggccctg ccggtctcct catgggccag aagttcccca agaacccagc cgcagaactc
      961 atcaaaatgc acaaatgtac tttccctggc tgcagcaaga tgtacaccaa aagcagccac
     1021 ctcaaggccc acctgcgccg gcacacgggt gagaagccct tcgcctgcac ctggccaggc
     1081 tgcggctgga ggttctcgcg ctctgacgag ctgtcgcggc acaggcgctc gcactcaggt
     1141 gtgaagccgt accagtgtcc tgtgtgcgag aagaagttcg cgcggagcga ccacctctcc
     1201 aagcacatca aggtgcaccg cttcccgcgg agcagccgct ccgtgcgctc cgtgaactag
     1261 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1321 aaggacgacc ttcaacccga gatgacgtcc cg
//
