LOCUS       sequence-277582-        4175 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1349
FEATURES             Location/Qualifiers
     source          1..4175
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggacac cgacctgttc atggaatgtg aggaggagga gttggagcca
       61 tggcagaaaa tcagtgatgt cattgaggac tctgtagttg aagattataa ttcagtggat
      121 aaaactacca cagctggcaa tcctttggtc cagcaaggtg gacagccact catcctgacc
      181 cagaatccag ccccaggtct gggcacaatg gttactcaac cagtattgag gcctgttcag
      241 gtcatgcaga atgccaatca tgtgactagt tcccctgtgg cctcacaacc aatatttatc
      301 actacgcagg gatttcctgt aaggaatgtc cggcctgtac aaaatgcaat gaatcaggtt
      361 gggattgtgc tgaacgtaca gcaaggccaa acggttagac caattacact agttccagcc
      421 ccaggtaccc agtttgttaa gccgacagtt ggagttccac aagtgttctc ccagatgacc
      481 cctgtgaggc caggctccac aatgcctgtg aggcccacca ccaacacctt caccaccgtc
      541 atcccggcca ctcttaccat tcgaagcacc gtcccacagt cccagtccca gcagaccaag
      601 tccactccca gcacttctac cactcccact gccacacagc caacctcact ggggcaacta
      661 gctgttcagt ctccaggcca gtcaaaccag accacgaatc ccaagctagc tccctccttc
      721 ccctctccac ctgcagtgag cattgccagc tttgtcactg tgaagcgacc tggtgttaca
      781 ggcgaaaata gcaatgaagt ggccaaattg gtgaataccc ttaacaccat cccttccctg
      841 ggccagagtc ctgggccagt ggtggtgtcc aacaacagct ctgctcatgg ctctcaaaga
      901 accagcggac ctgagtcttc aatgaaagtg acctcttcca tcccagtatt tgacctccag
      961 gatggtggac ggaaaatatg tccacgatgt aatgctcaat ttcgtgttac tgaagctttg
     1021 agaggtcaca tgtgttactg ttgcccagaa atggttgaat accagaagaa aggaaagtcc
     1081 ctggattcag aacccagtgt cccatcagca gcaaagcccc catcccctga gaaaacagct
     1141 cctgttgctt ccacaccctc ttctacacct attcctgctc tgtcaccgcc taccaaagta
     1201 ccagaaccaa atgagaacgt gggcgatgcc gtccagacca aactcattat gcttgtagat
     1261 gacttctact atggacggga tggtggcaaa gtagcccagc tcacaaattt ccctaaggtc
     1321 gccacatctt tccgatgccc acattgtacc aaaaggctaa aaaacaatat tcgattcatg
     1381 aaccatatga aacaccacgt agaactcgat cagcagaacg gtgaggtaga tggtcacact
     1441 atctgccagc actgttaccg ccagttttcc actcccttcc agcttcagtg ccacttggaa
     1501 aatgttcata gtccctatga atctactacc aagtgcaaga tctgtgaatg ggcgtttgaa
     1561 agtgagccac tatttctcca gcatatgaag gatactcata agcctggaga gatgccttat
     1621 gtttgccagg tgtgtcaata tcgctcctca ctctactctg aggtagatgt ccattttcgg
     1681 atgatccatg aggatacccg gcatctgctc tgcccttatt gcctgaaggt cttcaaaaat
     1741 ggcaatgcat tccaacagca ttacatgagg caccagaaga gaaatgttta tcactgcaac
     1801 aaatgccggc tgcagtttct ctttgccaag gacaaaattg aacacaagct tcaacaccat
     1861 aaaaccttcc gtaaacccaa gcagctggag ggcttgaaac caggcaccaa ggtgacaatc
     1921 cgggcttccc gagggcagcc acgaactgtt cctgtatcct ctaatgatac acctcccagc
     1981 gccttgcagg aggcagcacc gctgacctcc tcaatggacc ctctgcctgt cttcctttat
     2041 ccccctgtcc agcgcagcat ccagaagaga gctgttagga aaatgagtgt catgggccgg
     2101 cagacatgcc tggagtgcag cttcgagatc ccagacttcc ctaatcattt ccctacttac
     2161 gtacactgct ctctgtgtcg ctatagcacc tgctgttctc gagcttatgc caaccacatg
     2221 atcaacaatc atgttccacg gaagagcccc aagtatttgg ctttgtttaa aaattctgtg
     2281 agtggaatca agctggcctg cacttcatgt acctttgtta cctctgtggg cgatgctatg
     2341 gccaagcatt tggtattcaa cccctctcac agatccagca gcatcctgcc acggggactc
     2401 acttggatag ctcactcaag gcatggccag actcgtgacc gagtgcatga ccggaacgtg
     2461 aagaatatgt accctcctcc ttccttcccc actaacaaag ctgccactgt gaaatctgcg
     2521 ggggccaccc cagctgagcc tgaagagcta ctaactccct tagccccagc actcccatca
     2581 ccagcctcaa ctgcaacccc accaccaacc cccactcacc cgcaggcttt agcccttcca
     2641 ccgctggcta cagagggagc cgaatgtctg aatgttgatg atcaggatga agggagccca
     2701 gtcacccaag aacctgagct agcatcaggt ggtggtggta gtggtggagt tggcaaaaag
     2761 gagcagctgt ctgtgaagaa gcttcgagta gtactgtttg ctctatgctg caatacagaa
     2821 caggcagctg aacacttccg aaatccccag cgacgtattc gccgttggct tcgacgtttc
     2881 caggcctccc agggggagaa tctagagggc aaatatctga gctttgaggc agaagagaaa
     2941 ctggctgagt gggtgctaac ccagcgcgaa caacagctac ctgtaaatga ggagaccttg
     3001 ttccagaagg ccaccaaaat aggacgttct ttggaagggg ggtttaagat ctcctatgag
     3061 tgggctgtgc gtttcatgct gcggcaccac ctgactcccc atgcccggcg agctgtggcc
     3121 cacaccctac ctaaggatgt agcagagaat gcaggactct tcattgattt tgtacaacgg
     3181 cagattcaca accaggactt acccttgtct atgattgtgg ctattgatga gatctctttg
     3241 ttcctggata cagaggtgct gagcagtgat gatcgaaagg agaatgccct gcagacagtg
     3301 ggcacagggg aaccttggtg tgatgtagtc ctagccattc tggcagatgg cactgtcctt
     3361 cccaccctgg ttttctacag agggcagatg gatcagcctg ctaacatgcc agactccata
     3421 ttgctagagg caaaggagag tggctacagt gatgacgaga tcatggagct gtggtcaact
     3481 cgagtgtggc agaagcacac agcttgccag cgcagcaaag gcatgcttgt gatggactgt
     3541 catcgcactc acttgtcaga agaggtactg gctatgctta gtgcctctag cactttgcct
     3601 gcagtggtcc cagcaggctg tagctccaaa attcagccat tagatgtatg catcaaaaga
     3661 actgtcaaga acttcctgca taaaaaatgg aaggaacagg ctcgggaaat ggcagatact
     3721 gcatgtgatt ctgatgtcct gcttcagctg gtgcttgtct ggctgggtga agtgctaggt
     3781 gtcattgggg actgtccaga gctagttcag cgctccttcc tggtggctag tgttctgcct
     3841 ggccccgatg gcaacattaa ctcacctaca agaaatgctg acatgcagga ggagctaatt
     3901 gcctccctag aggagcaact gaagctgagt ggggaacatt ctgagtcttc cactccacga
     3961 cccagatcat ctcctgaaga gacaattgag cctgaaagtc ttcaccagct ctttgagggt
     4021 gaaagtgaga ccgagtcttt ctatggcttt gaagaagctg acctagatct gatggagatt
     4081 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     4141 ggaaaggacg attagcgttg acatacgtat tcgta
//
