LOCUS       sequence-277600-        1166 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1371
FEATURES             Location/Qualifiers
     source          1..1166
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaagaat tctaagtcaa ggccctgtgt attccagttt taaaggaggc
       61 tggaaatgca aggatcatac tgagatgctg caagaaaatc agggatgtat taggaaagta
      121 acagtctctc atcaagaagc cctggctcaa catatgaata tcagtactgt ggagaggccc
      181 tatggatgcc atgaatgtgg aaaaactttt ggtcgacgct tttccctggt gttacaccag
      241 aggactcata ctggagagaa accatatgca tgtaaggaat gtggcaaaac ctttagccag
      301 atttcaaacc ttgtgaaaca ccaaatgata catactggaa agaaacccca tgagtgtaag
      361 gactgtaata aaacattcag ttacctttca tttcttattg aacaccagag aacgcacact
      421 ggggagaaac cttatgaatg tactgagtgt ggaaaggcct ttagccgtgc ctccaacctc
      481 actcgacatc aaagaattca cataggaaag aaacaatata tatgtaggaa atgtggtaaa
      541 gcatttagca gtggctcaga actcattcgc caccagatta cacatactgg agagaaacct
      601 tatgaatgca ttgaatgtgg gaaggcattt cgccgtttct cacaccttac tcgacatcag
      661 agcatccata caaccaaaac cccgtatgaa tgtaatgaat gtaggaaagc tttccgttgt
      721 cactcattcc ttattaaaca tcagagaatt catgctggag aaaagctcta tgaatgtgat
      781 gaatgtggta aagttttcac ttggcatgca tcccttattc aacatacgaa gagtcacact
      841 ggagagaaac cctatgcgtg tgctgaatgt gataaagcct tcagccggag cttttccctc
      901 attctacatc agagaactca tactggagag aaaccctatg tatgtaaggt atgcaacaaa
      961 tccttcagct ggagctcaaa ccttgctaaa catcagagga cacacactct tgacaacccc
     1021 tatgaatatg aaaattcatt taattaccac tcattcctta ctgaacacca gtagtaatga
     1081 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1141 gaagcgacgt tcattggtgt ccgtaa
//
