LOCUS       sequence-277639-        1463 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1541
FEATURES             Location/Qualifiers
     source          1..1463
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaccctgt tgctacccac agctgccatc tgctccagca actgcatgag
       61 cagcgaatcc aaggcctgct ttgtgactgt atgttggtgg taaaaggagt ctgctttaaa
      121 gcgcataaga atgtcctggc agcattcagc cagtatttta ggagcctctt tcagaattct
      181 tcaagccaga agaatgatgt ttttcacttg gatgttaaaa atgtcagtgg catagggcag
      241 atcctggact tcatgtacac ttctcatcta gatcttaacc aggacaatat acaagtaatg
      301 ctggacacag cacagtgttt gcaagttcaa aatgttctga gtctgtgtca cacattttta
      361 aaatcagcca ctgtagtaca gccacctggc atgccttgta atagtacatt gtctctacaa
      421 agcaccctga ccccagatgc cacttgtgtt atcagtgaaa actacccccc tcatttactg
      481 caggaatgtt cagcagatgc acagcagaac aaaacgttgg atgaatcgca tccgcatgct
      541 tcaccatcag ttaatcgtca tcactccgca ggtgaaatct caaaacaagc tcctgatact
      601 tcagatggca gctgcacaga actgcctttc aaacagccaa attactatta caaactcaga
      661 aacttttaca gtaagcagta ccataaacac gcagctggtc ccagtcagga gagagttgtt
      721 gagcagcctt ttgctttcag cacctctaca gaccttacca cggtagagag ccagccttgt
      781 gccgtcagtc attctgaatg catcctggag tctcccgagc acttaccttc caacttcctg
      841 gcccagcctg tgaatgactc tgccccacac cctgagtcag acgccacatg ccaacaacct
      901 gtcaagcaga tgaggctcaa aaaggccatt catctgaaga agctcaattt cctgaagtca
      961 cagaaatacg cagagcaagt atctgaaccc aagtcagatg atggtttgac aaagaggttg
     1021 gaatctgcta gtaaaaatac cctagagaaa gctagcagcc aaagtgctga agaaaaagaa
     1081 agtgaagaag tcgtcagttg tgagaatttt aattgcatta gtgagacgga gaggcctgaa
     1141 gacccggctg ccctggaaga ccagtcccag acacttcagt cccagagaca atacgcgtgt
     1201 gaattatgcg ggaaaccttt taaacaccca agcaacttgg agcttcacaa acggtctcat
     1261 acaggtttgc agcctctggc gacgtccagc gtcacattat tattcactca ggagaaaaac
     1321 cacacttgtg tgacatctgt ggtcgagggt ttagtaactt cagtaattta gtaatgaact
     1381 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgag
     1441 cgggcatgcg ctaaggatct tga
//
