LOCUS       sequence-277642-         545 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1544
FEATURES             Location/Qualifiers
     source          1..545
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccttac tcaggtacgg ttgacattta gggatgtggc catagaattc
       61 tctcaggagg agtggaaatg cctggaccct gctcagagga tcttatacag ggacgtgatg
      121 ttggagaact actggaacct tgtttctctg ggactgtgtc attttgatat gaatattatc
      181 tccatgttgg aggaagggaa agagccctgg actgtgaaga gctgtgtgaa aatagcaaga
      241 aaaccaagaa cgccggaatg tgtcaaaggc gtggtcacag acctgctcag gagatggaaa
      301 cattggctgc tgctccttgg catctgctgc cccaaacctc atggaagagt ctccagccgt
      361 ctccgcctgt cgaggtccct cggccatttc ttccattctg cttttgccac tttcatgggg
      421 gtgtgtgata agcgagtggg ttctatcttc tagtaatgaa ctagtccgta acttgaaagt
      481 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaaatgcaat ttgctccggt
      541 gtggc
//
