LOCUS       sequence-277651-         644 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1557
FEATURES             Location/Qualifiers
     source          1..644
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggagacaa gaagagcccc accaggccga agcggcagcc gaagccgtcc
       61 tcggatgagg gttactggga ctgtagcgtc tgcaccttcc ggaacagcgc cgaggccttc
      121 aagtgcatga tgtgcgatgt gcggaagggc acctccaccc ggaaacctcg acctgtctcc
      181 cagttggttg cacagcaggt tactcagcag tttgtgcctc ctacacagtc aaagaaagag
      241 aaaaaagata aagtagaaaa agaaaaaagt gaaaaggaaa caactagcaa aaagaatagc
      301 cataagaaaa ccaggccaag attgaaaaat gtggatcgga gtagtgctca gcatttggaa
      361 gttactgttg gagatctgac agtcattatt acagacttta aggagaaaac aaagtcaccg
      421 cctgcatcta gtgctgcttc tgcagatcaa cacagtcaaa gcggctctag ctctgataac
      481 acagagagag gaatgtccag gtcatcttca cccagaggag aagcctcatc attgaatgga
      541 gaatctcatt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
      601 atatcttgtg gaaaggacga atatttgcgg tgagatgaga gcac
//
