LOCUS       sequence-277654-         455 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1558
FEATURES             Location/Qualifiers
     source          1..455
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggagacaa gaagagcccc accaggccga agcggcagcc gaagccgtcc
       61 tcggatgagg gttactggga ctgtagcgtc tgcaccttcc ggaacagcgc cgaggccttc
      121 aagtgcatga tgtgcgatgt gcggaagggc acctccaccc gggacagcaa ggaagggggg
      181 aagctggtgt cctactccac agccagtctt ggggttagag gaaccctgag aaatagagta
      241 ggtggtggca gctcagaaga gaagaaacag gctgaatacc tggcacctgg aagaagaagg
      301 aatatagtac acaggggagt tggcccagga cagagaagtg ggcctagttt aaaagaggct
      361 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
      421 ggaaaggacg agtcactcaa gagtaggttg gtggt
//
