LOCUS       sequence-277657-         518 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1559
FEATURES             Location/Qualifiers
     source          1..518
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggagacaa gaagagcccc accaggaaac ctcgacctgt ctcccagttg
       61 gttgcacagc aggttactca gcagtttgtg cctcctacac agtcaaagaa agagaaaaaa
      121 gataaagtag aaaaagaaaa aagtgaaaag gaaacaacta gcaaaaagaa tagccataag
      181 aaaaccaggc caagattgaa aaatgtggat cggagtagtg ctcagcattt ggaagttact
      241 gttggagatc tgacagtcat tattacagac tttaaggaga aaacaaagtc accgcctgca
      301 tctagtgctg cttctgcaga tcaacacagt caaagcggct ctagctctga taacacagag
      361 agaggaatgt ccaggtcatc ttcacccaga ggagaagcct catcattgaa tggagaatct
      421 cattagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
      481 tgtggaaagg acgatatccc acatggttct cgttcgcc
//
