LOCUS       sequence-277696-        1868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1628
FEATURES             Location/Qualifiers
     source          1..1868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggtttgta tggacaggct tgtccatctg taacttcatt aaggatgaca
       61 tctgaactgg agagcagcct aacgtctatg gactggttac cacagctcac catgagagca
      121 gccatccaaa aatctgatgc tacacaaaat gcacatggaa caggaatttc taagaagaat
      181 gcactccttg acccaaatac aactctggac caggaagaag tccaacagca caaagatggg
      241 aaacctccat acagttatgc cagcctcatt acatttgcaa ttaatagctc acccaaaaag
      301 aaaatgactt taagtgaaat ttatcagtgg atttgtgata acttcccata ttatagagag
      361 gctggcagtg gttggaagaa ttccatacga cataatctgt cattgaacaa atgtttcctt
      421 aaagtgcctc gatctaagga tgaccctgga aaggggtcct actgggcaat agacaccaat
      481 ccgaaggaag atgtgctgcc tactcggcca aagaagaggg cacgatctgt agaacgggta
      541 acattgtata acactgatca ggatggtagt gatagcccac gcagtagcct taacaacagt
      601 ctctcagacc agagtttggc atctgttaat ttgaacagtg ttggaagtgt gcatagttat
      661 acaccggtga caagccatcc agaatcagtc tctcaatcat taactcctca gcagcaacca
      721 cagtacaacc ttccagaaag agacaagcaa ctacttttct cagaatataa ttttgaagat
      781 cttagtgcct catttcggag cctttataag tcagtttttg agcagtcact tagtcaacaa
      841 ggtttgatga acatcccttc tgaatcttcc cagcagtccc acacttcatg tacctatcag
      901 cactctccca gcagtacagt gagcactcac ccacacagca accaaagcag cctgtccaac
      961 agtcatggca gtggcctcaa caccacaggc agtaattcgg ttgcacaggt ctcactgtct
     1021 cacccccaga tgcacacaca gccatctcca catcctcccc atcgaccgca tggtttaccg
     1081 cagcatccgc agcgttcccc acacccagca ccacacccac agcaacacag ccagctccag
     1141 tcccctcacc cccagcatcc ctctccacat caacacatac agcaccatcc gaaccatcag
     1201 catcagacgt taacacatca ggcaccccca cccccacaac aggtatcctg taattctggt
     1261 gtttcaaatg attggtatgc gacacttgat atgctaaaag aaagctgtcg aattgccagc
     1321 agtgttaatt ggtcagatgt agacctttca cagtttcaag gtctgatgga gagtatgaga
     1381 caggcagatc tcaagaactg gtctttagac caggttcagt ttgccgatct ttgttcttct
     1441 cttaatcagt tctttacaca aactggcctt atacattcac agagtaatgt tcaacaaaat
     1501 gtttgtcatg gtgccatgca tccaacaaaa ccttcccaac acattggaac aggaaatttg
     1561 tacatagatt ctaggcaaaa tctccctcct tcagtgatgc caccccctgg ttatcctcat
     1621 atcccacagg cactcagcac tccaggaaca acgatggcag gccatcacag agccatgaac
     1681 cagcagcaca tgatgccttc ccaagccttc cagatgcggc gttccctgcc tccagatgac
     1741 atccaggatg actttgattg ggattcaatt gtgtagtaat gaactagtcc gtaacttgaa
     1801 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaagcgag ccgagaccgt
     1861 cgggtaga
//
