LOCUS       sequence-277711-        1652 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1642
FEATURES             Location/Qualifiers
     source          1..1652
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggattcagt gacctttgac gatgtggctg tggacttcac ccaggaggag
       61 tggactttac tggactcaac tcagagaagc ctctacagtg acgtgatgct ggagaactac
      121 aagaacctgg ccacagtagg aggtcagatc atcaaaccca gtctaatctc ttggttggaa
      181 caagaagagt caaggacagt tcagggagga gttctccaag gatgggaaat gcgacttgaa
      241 acccagtggt ctatacttca gcaggacttt ttgaggggtc agacatccat tgggatacaa
      301 ttggaaggaa aacacaatgg aagggaactc tgtgactgtg agcaatgtgg agaagtcttc
      361 agtgaacact catgccttaa gacgcacgtg agaactcaaa gtacagggaa cactcatgac
      421 tgtaatcagt atggaaaaga tttccttacc ctgtgtgaga aaacctctac tggtgagaaa
      481 ctttctgagt ttaatcagag tgaaaaaatc ttcagcctga caccaaatat tgtataccag
      541 agaactagca cacaagaaaa gtcatttgaa tgtagtcact gtggaaaatc cttcattaat
      601 gagtcatacc ttcaggcaca tatgagaact cacaatggag aaaaactcta cgaatggagg
      661 aattatgggc caggttttat tgactctaca agcctttctg tgcttataga aaccctcaat
      721 gcaaaaaagc cctacaaatg taaggaatgt ggaaaaggct atagataccc agcctacctc
      781 agtattcaca tgcgaaccca cactggggag aaaccatatg aatgtaagga atgtgggaaa
      841 gccttcaatt attccaactc atttcagata catggaagaa ctcacactgg agagaaaccc
      901 tatgtatgta aggaatgtgg gaaagccttc actcagtact cgggccttag tatgcatgta
      961 cgatctcaca gtggagacaa gccctatgaa tgtaaggaat gtgggaaatc cttccttaca
     1021 tcctcacgcc ttattcaaca tataagaact cacactggag agaagccttt tgtatgtgtt
     1081 gaatgtggga aagcctttgc agtttcctca aatcttagtg gacatttgag aactcacact
     1141 gaagagaagg cctgtgagtg taagatatgt gggaaagtat ttgggtatcc ctcatgtctt
     1201 aataatcaca tgcgaacgca cagtgcccag aaaccataca cctgtaagga atgtgggaag
     1261 gcttttaact attccaccca ccttaaaatt cacatgcgaa tccacactgg agaaaaaccc
     1321 tatgagtgta aacaatgtgg aaaggccttc agtcattcca gttcatttca aatacatgaa
     1381 aggactcaca ctggagagaa accctatgaa tgcaaggagt gtgggaaagc cttcacgtgt
     1441 tccagttcct ttagaattca tgaaaaaact cacacagaag agaaacccta taaatgtcag
     1501 caatgcggga aagcttacag tcatccccgt tcacttcgaa gacatgaaca aattcactag
     1561 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1621 aaggacgact agtgcactca tccgtatgat gc
//
