LOCUS       sequence-277714-         458 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1643
FEATURES             Location/Qualifiers
     source          1..458
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagctgc tgatttgtcc catggacatt atctttctgg ggacccagtt
       61 tgccttcatg aagaaaagac accagcagga agaatagtgg ctgactgcct aacagattgt
      121 tatcaggatt cagtgacctt tgacgatgtg gctgtggact tcacccagga ggagtggact
      181 ttactggact caactcagag aagcctctac agtgacgtga tgctggagaa ctacaagaac
      241 ctggccacag taggaggtca gatcatcaaa cccagtctaa tctcttggtt ggaacaagaa
      301 gagtcaagga cagttcaggg aggagttctc caaggaagga aaacacaatg gaagggaact
      361 ctgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
      421 tgtggaaagg acgaagcgct ccgttcccta agttaacc
//
