LOCUS       sequence-277738-         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1701
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacgattc cattaataat agattttcct atgtaactca tgaagacatc
       61 tgtaattgct ttaatggtga tacacttttg gccattcagg caccttctgg tacacaactg
      121 gaggtaccca ttccagaaat gggtcagaat ggacaaaaga aataccagat caatctaaag
      181 agtcattcag gacctatcca tgtgctgctt ataaataaag agtcgagttc atctaagccc
      241 gtggtttttc ctgttccccc acctgatgac ctcacacagc cttcctccca gtccttgact
      301 ccagtgactc cacagaaatc cagcatggca actcaaaatc tgcctgagca acatgtctct
      361 gaaagaagcc aggctctgca gcagacatca gctacagata tatcttcagc aggatctatt
      421 agtggagata tcattgatga gttaatgtct tctgacgtgt ttcctctctt aaggctttct
      481 cctaccccgg cagatgacta caactttaat ttagatgata acgaaggagt ttgtgatctg
      541 tttgatgtcc agatactaaa ttattagtaa tgaactagtc cgtaacttga aagtatttcg
      601 atttcttggc tttatatatc ttgtggaaag gacgacccgc gactactaca cgaaagccc
//
