LOCUS       sequence-277741-        3401 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1707
FEATURES             Location/Qualifiers
     source          1..3401
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcacacct tgggcccacc ccacctccac atagccttaa ttacaaatca
       61 gaggacaggc ttagtgagca agactggcca gcatatttca aggtcccatg ttgtggggtt
      121 gatacatctc aaattgagtc agaagaggca gaagtggatg tgagagaaag agagacacag
      181 agagacagag agccaaagag ggcaagagac ttgactttaa gagactcctg tactgacaac
      241 tccatgcagt tcggaaccag aacgactacg gctgaaccag ggttcatggg gacatggcaa
      301 aacgctgata ctaacctctt attcagaatg tcccaacagg ccatccgttg cacactggta
      361 aactgcacat gtgaatgttt tcagccaggg aagattaacc tgaggacttg tgatcagtgt
      421 aaacatggct gggtggcaca tgccttggat aagctcagca cgcagcacct gtaccacccc
      481 acccaagtgg agattgtgca gtccaacgtc gtgtttgaca tcagcagcct gatgctctat
      541 gggacacaag cagtgcctgt gcggctaaag atcctgctgg accgtctctt cagcgtcctg
      601 aagcaagagg aggtactgca catactgcac ggccttggct ggactctgcg ggactatgtc
      661 cgaggataca tccttcagga tgctgctggc aaggtgctgg accgctgggc catcatgtct
      721 cgagaagagg aaatcatcac ccttcagcag tttctgcggt ttggagaaac caaatccatt
      781 gtggagctga tggcaattca ggagaaagaa gggcaggccg tggctgtacc atcttcaaag
      841 acagactcag atataaggac tttcattgag agcaataatc gcaccaggag tcccagcctc
      901 cttgctcact tagagaacag caatccttcc agcattcatc acttcgaaaa catcccaaac
      961 agccttgcat ttctgcttcc attccagtac ataaaccctg tctcagcacc actgctaggg
     1021 ttgcctccaa atgggctact gttagagcaa ccagggttga ggctgcggga acccagcctt
     1081 tcaactcaga atgaatataa tgagagcagc gaatccgaag tttctcccac accttataag
     1141 aatgatcaaa cacccaatag aaatgccctg accagcatta ctaatgtgga gcccaaaacc
     1201 gagccagcct gtgtctctcc cattcagaat tctgccccag tcagtgatct aaccaaaact
     1261 gaacacccaa aaagctcatt ccggattcat cggatgagaa ggatggggtc agcctctagg
     1321 aaaggaagag tgttctgtaa tgcatgtggg aagacattct atgacaaagg tactctcaaa
     1381 attcattaca atgctgttca cctgaagatc aaacatcgat gcaccattga aggttgcaac
     1441 atggtcttta gctccctccg aagtcgtaat cgccacagtg caaaccccaa tcctcgcctt
     1501 cacatgccta tgctaaggaa taaccgagat aaagatttaa ttcgggccac ctcaggagct
     1561 gccacccctg tcatagcaag tacaaaatca aatctggcac tcacaagccc tggccgaccc
     1621 ccaatgggtt ttaccactcc ccctctagac cctgtcttgc aaaatcctct ccctagccag
     1681 ctagtatttt ctgggctaaa gactgtacaa ccagttcctc cattttatag aagtttactc
     1741 actccagggg aaatggtgag tcctccaacc tccctcccaa ccagtcccat cattccaacc
     1801 agtggtacca tagagcagca ccccccgcca ccctctgagc cagtagtgcc agcagtgatg
     1861 atggccaccc atgagcccag tgctgacctg gcacccaaga aaaagcccag gaagtcaagc
     1921 atgcctgtga agattgagaa ggaaattatt gataccgccg atgagtttga tgatgaagat
     1981 gatgacccca atgatggtgg agctgtggtc aatgacatga gccatgacaa tcattgtcac
     2041 tcccaagagg agatgagccc aggcatgtct gtgaaggact tttctaagca taacaggacc
     2101 cggtgcattt caaggactga aataaggagg gccgacagca tgacttctga agaccaagaa
     2161 cctgagcggg actatgagaa cgagtctgag tcttcggagc ccaaactggg cgaggaatcc
     2221 atggaagggg atgagcacat tcacagcgaa gtgagtgaaa aagtcctgat gaatagtgag
     2281 aggcctgatg agaaccacag tgagccctct caccaggacg tcatcaaggt gaaggaagaa
     2341 tttacagacc ccacttacga catgttttac atgagccagt atggactgta caatggtggg
     2401 ggtgccagca tggccgcctt gcatgagagc tttacatcgt ctctgaatta tggcagccct
     2461 caaaagttct ccccagaagg tgacctatgt tctagcccag accccaaaat ctgttatgtg
     2521 tgcaagaaga gtttcaaaag ctcctacagt gtgaaacttc actacaggaa cgttcacttg
     2581 aaagagatgc acgtctgcac agtggctggt tgcaatgctg cattcccctc tcgccgaagc
     2641 cgagacagac acagtgccaa cataaaccta catcgtaaac tgttgaccaa agaactcgat
     2701 gacatgggcc tggactcgtc gcagccctcc cttagcaagg acctccgcga tgaatttttg
     2761 gtgaagatat atggtgccca gcaccccatg gggctcgatg tcagggaaga cgcctcctct
     2821 cccgcaggga ctgaagactc ccacctgaac gggtatggga gaggcatggc agaggactac
     2881 atggtccttg acttgagcac cacctccagc ctccagtcca gcagcagtat ccattcctcc
     2941 agagaatccg acgcaggcag cgatgagggg attcttctcg atgacattga cggggcgagt
     3001 gacagtgggg agtcggcaca caaggccgag gcccctgccc tccctggcag cctaggggct
     3061 gaagtttcag gatctcttat gttcagcagc ttgtctggga gcaatggtgg gatcatgtgc
     3121 aacatttgcc acaaaatgta cagcaacaag gggaccctga gagtgcacta caaaactgtg
     3181 catttgagag aaatgcacaa gtgcaaagtc ccaggttgca atatgatgtt ttcctctgta
     3241 cgaagccgaa atcggcacag tcagaaccct aatctccaca aaaacattcc cttcacttca
     3301 gtagattagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     3361 tcttgtggaa aggacgagcc aaataaatcc gcgcattact c
//
