LOCUS       sequence-277747-         620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1728
FEATURES             Location/Qualifiers
     source          1..620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggactatt ggcattcagg gatgtggctc tagaattctc tccagaggag
       61 tgggaatgcc tggacccagc tcagcggagt ttgtataggg atgtgatgtt agagaactac
      121 agaaacctga tctcccttgg tgaggatagc ttcaatatgc aattcctatt tcacagtctt
      181 gctatgtcta agccagaact gatcatctgt ctggaggcaa ggaaagagcc ctggaacgtg
      241 aacacagaga agacagccag acactcagtt ttgtcttctt atcttactga agacattttg
      301 ccagagcagg gcctgcaagt ttcattccaa aaagtgatgc tgagaagata tgaaagatgt
      361 tgtcttgaga aattacgctt aaggaatgac tgggaaattc catgtgaaga tgtgcttgct
      421 tcccctttgc cttctgccat gattctaagt ttcctgaggc ctccccagaa gcagaagcat
      481 gtaaagccca cagaaccggt ccagtcgtcg accatcgagc tgctttagta atgaactagt
      541 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgagaac
      601 gctacgtcag ccttgattac
//
