LOCUS       sequence-277756-        1448 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1809
FEATURES             Location/Qualifiers
     source          1..1448
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccatgag tgagctgggc actcggaagc ccagcgatgg cactgtttct
       61 catctgctca atgtggtgga gagtgagctt caggcaggga gggaaaaagg cgaccctacg
      121 gagaagcaac ttcagatcat cctggaggat gcacctctct ggcagagatt caaggaagtc
      181 actaatgaga tgattgtgac caagaatggc agacggatgt ttccagtcct aaagattagt
      241 gtcacagggt tggaccccaa tgccatgtac tccctcctgc tggactttgt ccctacggac
      301 agtcaccgct ggaagtacgt caacggggaa tgggtgcccg ctggcaagcc agaggtctcc
      361 agccacagct gcgtctacat tcacccggac tcccccaact ttggggccca ctggatgaaa
      421 gctcccatct ccttcagcaa agtgaagctg accaacaagc tcaatggagg cgggcagata
      481 atgttgaatt ctctgcataa atatgaaccc caggttcaca tagtgcgtgt tggaagtgcc
      541 catcgaatgg taacaaactg ctccttccct gaaacccagt tcatagccgt gactgcctat
      601 cagaatgagg agataacggc tctcaaaatc aagtacaatc cttttgccaa agccttcttg
      661 gatgccaagg aaagaaatca cctaagagac gtaccggagg ctatctctga gagccagcat
      721 gtgacctatt ctcacttggg aggctggatc ttttccaatc cagatggagt gtgcacagca
      781 ggaaactcca attaccagta tgccgctcct ctgcctctgc ctgctcccca cacccaccat
      841 ggctgtgagc actattcggg tctccgagga caccggcagg ctccctaccc ttctgcgtac
      901 atgcacagaa accattctcc ctcagtgaat ttgatagaaa gctcaagcaa taatctgcaa
      961 gttttctcgg gacctgacag ctggacttcc ttatcctcca caccccatgc cagcatcctg
     1021 tctgtacccc acaccaacgg accaatcaat ccagggccca gcccctaccc gtgcctgtgg
     1081 accatcagca atggtgccgg aggccccagt gggccaggcc cggaggtgca cgccagcacc
     1141 ccaggagcat ttctcctcgg aaacccagct gtgacttcac ccccttctgt gctctccacc
     1201 caagcaccca cttcggctgg tgtggaggtt ctgggggagc cctcgctaac cagcattgct
     1261 gtgtccacct ggacagcagt ggcctcgcat cccttcgcgg gctggggtgg cccaggagcg
     1321 ggtgggcacc attctccttc ctcactggat ggttagtaat gaactagtcc gtaacttgaa
     1381 agtatttcga tttcttggct ttatatatct tgtggaaagg acgattcgtt tctggataaa
     1441 gagaaact
//
