LOCUS       sequence-27776-e         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Chen Lf , Fischle W, Verdin E, Greene WC
  TITLE     Duration of nuclear NF-kappaB action regulated by reversible 
            acetylation.
  JOURNAL   Science. 2001 Aug 31. 293(5535):1653-7.
  PUBMED    11533489
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2001 Aug 31. 293(5535):1653-7."
COMMENT     Sequence Label: T7-RelA
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..29
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           98..137
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             173..205
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
       61 tccatagaag acaccgggac cgatccagcc tccgggggat cttggtggcg tgaaactccc
      121 gcacctcttc ggccagcgcc ttgtagaagc gcgtatggct tcgtggggat ctatggctag
      181 catgactggt ggacagcaaa tgggtcgcgg atccgacgaa ctgttccccc tcatcttccc
      241 ggcagagcca gcccaggcct ctggccccta tgtggagatc attgagcagc ccaagcagcg
      301 gggcatgcgc ttccgctaca agtgcgaggg gcgctccgcg ggcagcatcc caggcgagag
      361 gagcacagat accaccaaga cccaccccac catcaagatc aatggctaca caggaccagg
      421 gacagtgcgc atctccctgg tcaccaagga ccctcctcac cggcctcacc cccacgagct
      481 tgtaggaaag gactgccggg atggcttcta tgaggctgag ctctgcccgg accgctgcat
      541 ccacagtttc cagaacctgg gaatccagtg tgtgaagaag cgggacctgg agcaggctat
      601 cagtcagcgc atccagacca acaacaaccc cttccaagtt cctatagaag agcagcgtgg
      661 ggactacgac ctgaatgctg tgcggctctg cttccaggtg acagtgcg
//
