LOCUS       sequence-277762-         482 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1822
FEATURES             Location/Qualifiers
     source          1..482
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggaccact gacatttatg gatgtggcca tagagttctc tttggaggag
       61 tggcagtgcc tggacactgc acagcggaat gtatataggc atgtgatgtt agagaactac
      121 agaaacctgg ttttcttgga cctgattacc tgtctggaac aaggaaaaga gccctggaat
      181 atgaagagac atgagatggt ggtagccaaa cattcagctg tagcatttac ctttgatctc
      241 agtggactca acattttgtc attccaaagt atcctgccat ttgtttcagc actttatgtc
      301 atgggaaaca gaaaccagtg tctgcaaaag cacctagaag ccagaaataa agatctctgt
      361 gttctcgttt tgcccaagac ctttggctag taatgaacta gtccgtaact tgaaagtatt
      421 tcgatttctt ggctttatat atcttgtgga aaggacgatg cgagacattc acagggagca
      481 ca
//
