LOCUS       sequence-277774-        2402 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1871
FEATURES             Location/Qualifiers
     source          1..2402
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggacgg aggagcagcg agtcaagatg agagttcagc cgcggcggca
       61 gcagcagcag actcaagaat gaacaatccg tcagaaacca gtaaaccatc tatggagagt
      121 ggagatggca acacaggcac acaaaccaat ggtctggact ttcagaagca gcctgtgcct
      181 gtaggaggag caatctcaac agcccaggcg caggctttcc ttggacatct ccatcaggtc
      241 caactcgctg gaacaagttt acaggctgct gctcagtctt taaatgtaca gtctaaatct
      301 aatgaagaat cgggggattc gcagcagcca agccagcctt cccagcagcc ttcagtgcag
      361 gcagccattc cccagaccca gcttatgcta gctggaggac agataactgg gcttactttg
      421 acgcctgccc agcaacagtt actactccag caggcacagg cacaggcaca gctgctggct
      481 gctgcagtgc agcagcactc cgccagccag cagcacagtg ctgctggagc caccatctcc
      541 gcctctgctg ccacgcccat gacgcagatc cccctgtctc agcccataca gatcgcacag
      601 gatcttcaac aactgcaaca gcttcaacag cagaatctca acctgcaaca gtttgtgttg
      661 gtgcatccaa ccaccaattt gcagccagcg cagtttatca tctcacagac gccccagggc
      721 cagcagggtc tcctgcaagc gcaaaatctt ctaacgcaac tacctcagca aagccaagcc
      781 aacctcctac agtcgcagcc aagcatcacc ctcacctccc agccagcaac cccaacacgc
      841 acaatagcag caaccccaat tcagacactt ccacagagcc agtcaacacc aaagcgaatt
      901 gatactccca gcttggagga gcccagtgac cttgaggagc ttgagcagtt tgccaagacc
      961 ttcaaacaaa gacgaatcaa acttggattc actcagggtg atgttgggct cgctatgggg
     1021 aaactatatg gaaatgactt cagccaaact accatctctc gatttgaagc cttgaacctc
     1081 agctttaaga acatgtgcaa gttgaagcca cttttagaga agtggctaaa tgatgcagag
     1141 aacctctcat ctgattcgtc cctctccagc ccaagtgccc tgaattctcc aggaattgag
     1201 ggcttgagcc gtaggaggaa gaaacgcacc agcatagaga ccaacatccg tgtggcctta
     1261 gagaagagtt tcttggagaa tcaaaagcct acctcggaag agatcactat gattgctgat
     1321 cagctcaata tggaaaaaga ggtgattcgt gtttggttct gtaaccgccg ccagaaagaa
     1381 aaaagaatca acccaccaag cagtggtggg accagcagct cacctattaa agcaattttc
     1441 cccagcccaa cttcactggt ggcgaccaca ccaagccttg tgactagcag tgcagcaact
     1501 accctcacag tcagccctgt cctccctctg accagtgctg ctgtgacgaa tctttcagtt
     1561 acaggcactt cagacaccac ctccaacaac acagcaaccg tgatttccac agcgcctcca
     1621 gcttcctcag cagtcacgtc cccctctctg agtccctccc cttctgcctc agcctccacc
     1681 tccgaggcat ccagtgccag tgagaccagc acaacacaga ccacctccac tcctttgtcc
     1741 tcccctcttg ggaccagcca ggtgatggtg acagcatcag gtttgcaaac agcagcagct
     1801 gctgcccttc aaggagctgc acagttgcca gcaaatgcca gtcttgctgc catggcagct
     1861 gctgcaggac taaacccaag cctgatggca ccctcacagt ttgcggctgg aggtgcctta
     1921 ctcagtctga atccagggac cctgagcggt gctctcagcc cagctctaat gagcaacagt
     1981 acactggcaa ctattcaagc tcttgcttct ggtggctctc ttccaataac atcacttgat
     2041 gcaactggga acctggtatt tgccaatgcg ggaggagccc ccaacatcgt gactgcccct
     2101 ctgttcctga accctcagaa cctctctctg ctcaccagca accctgttag cttggtctct
     2161 gccgccgcag catctgcagg gaactctgca cctgtagcca gccttcacgc cacctccacc
     2221 tctgctgagt ccatccagaa ctctctcttc acagtggcct ctgccagcgg ggctgcgtcc
     2281 accaccacca ccgcctccaa ggcacagtag taatgaacta gtccgtaact tgaaagtatt
     2341 tcgatttctt ggctttatat atcttgtgga aaggacgacg cagggctcca aatagacgac
     2401 gc
//
