LOCUS       sequence-27778-e         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Kobashigawa Y, Kumeta H, Ogura K, Inagaki F
  TITLE     Attachment of an NMR-invisible solubility enhancement tag using a 
            sortase-mediated protein ligation method.
  JOURNAL   J Biomol NMR. 2009 Mar . 43(3):145-50.
  PUBMED    19140010
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biomol 
            NMR. 2009 Mar . 43(3):145-50."
COMMENT     Sequence Label: pGBHPS-SortA
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(515..538)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(539..556)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cttctttcgg gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagatt
       61 atttgacttc tgtagctaca aagattttac gtttttccca aacgcctgtc ttttcattgt
      121 aatcatcaca agtaattaat gttaattgtt tatctttacc tttttgttca tctagaactt
      181 ctacatctgt tggcttaaca tctcttatac ttgtcatttt atacttacgt gtttcattac
      241 caactttaaa gtacaccata ctaccttttt tggctgcttt aagatttgta aattgatagt
      301 tcggacggtc aatgaaagtg tgtcctgcaa ttgaaatatt ttgatcatct agtgattcat
      361 tttcttctgc aaagcttaca cctctattta attgttcagg tgttgctggt cctggatata
      421 ctggttcttt aatatcagca tctggaattt caatatagcc tgccactttt gatttatctt
      481 tcggaatttg aggtttagct tgggatccca tatgcgggcc ctgaaacagc acttccagat
      541 gatgatgatg atgatggcta tgttcggtta ccgtgaaggt tttggtagcg tcgtcgtagg
      601 tccattcacc gtcaacaccg ttgtcgttag cgtactgttt gaaaactttt tccgcggtag
      661 cagcgtcaac agcttcggtg gtggtttcac ctttca
//
