LOCUS       sequence-27779-e         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Kobashigawa Y, Kumeta H, Ogura K, Inagaki F
  TITLE     Attachment of an NMR-invisible solubility enhancement tag using a 
            sortase-mediated protein ligation method.
  JOURNAL   J Biomol NMR. 2009 Mar . 43(3):145-50.
  PUBMED    19140010
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biomol 
            NMR. 2009 Mar . 43(3):145-50."
COMMENT     Sequence Label: pGBMCS-SortA
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(511..672)
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     RBS             685..707
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cttctttcgg gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagttt
       61 gacttctgta gctacaaaga ttttacgttt ttcccaaacg cctgtctttt cattgtaatc
      121 atcacaagta attaatgtta attgtttatc tttacctttt tgttcatcta gaacttctac
      181 atctgttggc ttaacatctc ttatacttgt cattttatac ttacgtgttt cattaccaac
      241 tttaaagtac accatactac cttttttggc tgctttaaga tttgtaaatt gatagttcgg
      301 acggtcaatg aaagtgtgtc ctgcaattga aatattttga tcatctagtg attcattttc
      361 ttctgcaaag cttacacctc tatttaattg ttcaggtgtt gctggtcctg gatatactgg
      421 ttctttaata tcagcatctg gaatttcaat atagcctgcc acttttgatt tatctttcgg
      481 aatttgaggt ttagcttggg atcccatatg ttcggttacc gtgaaggttt tggtagcgtc
      541 gtcgtaggtc cattcaccgt caacaccgtt gtcgttagcg tactgtttga aaactttttc
      601 cgcggtagca gcgtcaacag cttcggtggt ggtttcacct ttcagggttt taccgttcag
      661 gatcagtttg tactccatgg tatatctcct tcttaaagtt aaacaaaatt atttctagag
      721 gggaattgtt atccgct
//
