LOCUS       sequence-277819-         554 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1984
FEATURES             Location/Qualifiers
     source          1..554
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccttac tcaggggcaa ttgtcattca gcgatgtggc catagaattc
       61 tctcaggagg agtggaaatg cctggaccct gggcagaaag ctttatacag ggacgtgatg
      121 ttagagaact acaggaacct ggtctcccta gatttttcac ttctggcctg ggacgcgcgc
      181 gtcttcaggg tggaagcctg gcgcgcgtcc ggaggtgctg aggagccaac cggcccaaac
      241 tttgtgggaa atgactcccc tctgccctcg cccctcgctc tgctaccatt tccttccgtt
      301 tctgcttcgc ttggcgatgc aaaacgcgcg aggctcacgg cagagggccg aggccacggg
      361 actctcaggg ccgggcccgc ccctcggccg cgcagtgcgg cgccgcacgc cccggacgct
      421 gtccagcgtt ggccgcctga gcagaggctg ccgcggagct agtaatgaac tagtccgtaa
      481 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga ttccacctct
      541 agttgagagg taac
//
