LOCUS       sequence-27782-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  TITLE     TRONO lab plasmids
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRRL_H1shmp53_PGK_eGFP_Teto
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        277..367
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     288..307
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_feature    451..568
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgtttccgga ncncgcccag agcagccgcg tccctgcgca aacccagggc tgccttggaa
       61 aaggcgcaac cccaaccccg tggaattcga tatcaagctt ctcgagcggc cgccagtgtg
      121 atggatatct gcagaattcg cccttcgcgc gctcgatctg acgtcatcaa cccgctccaa
      181 ggaatcgcgg gcccagtgtc actaggcggg aacacccagc gcgcgtgcgc cctggcagga
      241 agatggctgt gagggacagg ggagtggcgc cctgcaatat ttgcatgtcg ctatgtgttc
      301 tgggaaatca ccataaacgt gaaatgtctt tggatttggg aatcttataa gttctgtatg
      361 agaccacaga tcccactaca agtacatgtg tatcaagaga aatacacatg tacttgtagt
      421 gtttttctcg aggctagtct cgtgatcgat aaaattttga atttttgtaa tttgtttttg
      481 taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta
      541 ccccccaatc cccccttttc ttttaaaagt taaccgatac cgtcgagatc cgttcactaa
      601 tcgaatggat ctgtctctgt ctctctctcc accttcttct tctattcctt cgggcctgtc
      661 gggtcccctc ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac
      721 tctattcact atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat
      781 cattatgaat aattttatat accacagcca atttgttatg ttaaaccaat tccacaaact
      841 tgcccattta tctanttcca ataattcttg ttcattcttt tcttgctggt tttgcnattc
      901 ttcaatta
//
